Clinical, computed tomography scan and MRI evaluation of osteodisc allograft impregnated with mesenchymal stem cells for replacement of normal cervical disc in dog

13^th International Veterinary Congress

May 02-03, 2019 | London, UK

Davood Sharifi, Shaghayegh Rafatpanah, Mohammadreza Golbakhsh, Sarang Soroori, Fatemeh Aramesh and Alireza Bashiri

University of Tehran, Iran

Scientific Tracks Abstracts: J Vet Sci Technol

Abstract :

Statement of the problem: Spinal arthroplasty offers an exciting alternative to fusion for the treatment of degenerative disc disease. Currently, the main indication for disc prosthesis in small animals is the treatment of disc-associated cervical spondylomyelopathy in dogs, also known as Disc-Associated Wobbler Syndrome (DAWS). The objective of this study was to evaluate the immediate postoperative recovery and the short- and intermediate-term follow-up of dogs treated with Allograft osteodisc cervical disc replacement over 6 months being evaluated with CT Scan and MRI.

Methodology & Materials: The complete cervical vertebrae from two medium size mongrel dogs cadaver free from systemic diseases, freshly were collected. The total of 3 discs along with end-plate and either side vertebral bodies were removed from each cadaver and transplanted to 3 other adult â?? large male mixed breed (25±30 Kg.bw) after removal of 2nd normal vertebral disc in each one under restricted aseptic condition and stabilized with double 2.7 mm cancellous screw. Allograft osteodisc was impregnated with 1 ml broth of autologous mesenchymal stem cells before replacement (10-10) in first 3 dogs whereas the rest of 3 dogs used as control one. CT Scan and MRI were done before and after replacement of allograft osteodisc and at end of study in 6 months. Five main CT Scan and MRI parameters including, moderate dehydration rate, mild rate of resorption of replaced disc materials, decreased signal of replaced disc materials in T1-T2 images, rate of protrusion of replaced disc, osteodisc density, osteophytes & calcification of allograft osteodisc, dislocation and distance of grafted disc beside complications and tracing infection & facture.

Findings: All dogs had immediate postoperative recovery with good degree of distraction in the Immediate postoperative CT Scan. In the majority of dogs, the amount of distraction decreased slightly over-time; this except for one dog was not clinically relevant. In this one dog CT Scan data showed one grafted osteodisc displacement due to dislocation of tissue grafted. But it plays a role in reconstruction or maintenance of intervertebral functional spinal unit, clinical outcomes indicated that using osteodisc allograft for cervical disk replacement is reliable. Heterogenous density with one fracture complication with variable changes in disc distance were prominent MRI and CT Scan findings.

Conclusion & Significance: Cervical disc arthroplasty appears to be a well-tolerated surgical technique; Short- and Intermediate-term results are promising. Long-term follow-up studies are underway. We feel this surgery provides a better outcome for dynamic CSM; it is less invasive, requires minimal post-op care and hospitalization, reduces post-op pain and discomfort, has a lower mortality rate and a lower cost to the owners.

Recent Publications

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2. Lam S K, Xiao J, Ruan D, Ding Y, Lu W W, Luk K D (2012) The effect of remodeling on the kinematics of the malpositioned disc allograft transplantation. Spine (Phila Pa 1976). 37(6):E357â??66.

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