alexa Cloning And Sequencing Of The Rap-1α1 Gene From Mexican Isolates Of Babesia Bigemina
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

7th International Veterinary Congress
September 04-05, 2017 | Paris, France

Julio V Figueroa, Jose J Lira, Patricia Vargas, Carmen Rojas and J Antonio Alvarez
National Research Center for Veterinary Parasitology, Mexico
Posters & Accepted Abstracts: J Vet Sci Technol
DOI: 10.4172/2157-7579-C1-025
Abstract
The Babesia bigemina RAP-1 antigen is a 58 kDa protein located in the merozoite roptries of the protozoan parasite, which participates in the invasion to the bovine erythrocyte. rap-1 sequences of B. bigemina isolates from Mexico, Brazil, Argentina, Uruguay and Puerto Rico are known. However, the degree of conservation of rap-1 sequence in different geographic isolates of Mexico is not known. The aim of this study was to analyze rap-1α1 sequences in 8 different isolates of B. bigemina from Mexico. The methodology included the extraction of genomic DNA from B. bigemina-infected erythrocytes; PCR amplification of rap- 1α1 using rap-1F and rap-1R as primers; Cloning the amplified fragment (1440 bp) into TOPO 10 vector and transforming competent E. coli cells; Selection of clones and purification of recombinant plasmids; Sequencing and analysis of rap-1α1 from the B. bigemina isolates, obtained from at least 3 recombinant clones per isolate, sequencing the inserts in both directions; and sequence alignments and assembling with the CLC application genomic workbench 4.8. The identity of the rap-1α1 sequences was determined by a homology search with BLAST tools. The results showed amplification of rap-1α1 in all the B. bigemina isolates. The comparative analysis of nucleotide sequences revealed a high degree of conservation (98-99% identity) between the rap-1α1 genes of the different Mexican isolates, compared to the reference rap-1α1 sequence. BLASTX analysis revealed identities up to 99% in the deduced amino acid sequences of RAP-1 compared to the sequence of the reference isolate. The high degree of conservation in rap-1α1 sequences among geographically distant Mexican isolates suggests that there is no strong bovine immune pressure that translates into genetic variation of this particular gene. The RAP-1 antigen is a viable candidate for inclusion in a diagnostic test for bovine babesiosis caused by B. bigemina in Mexico.
Biography

Julio V Figueroa is currently working as a researcher and head of the National Research Center for Veterinary Parasitology, INIFAP, in Jiutepec, Morelos, Mexico. He obtained his Veterinary Medicine Degree from the State of Mexico Autonomous University in Toluca, Mexico, and the MSc and PhD degrees in Veterinary Pathology and Microbiology at the University of Columbia-Missouri, in Columbia, MO, USA. He has conducted research on tick borne diseases of cattle during the past 30 years and has published over 70 research papers in peer reviewed international journals.

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version