Comet Assay For Testing The Neurotoxic Effect Of Orally Administered Propionic Acid And Induced Gut Bacterial Imbalance: Protective Potency Of Carnosine And Carnitine | 16470
Journal of Molecular Biomarkers & Diagnosis
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Comet assay is a quick method for assessing DNA damage in individual cells. It allows the detection of single
and double DNA strand breaks which represent the direct effect of some damaging agent. This work aims to use comet
standard quantification models to compare the neurotoxic effect of orally administered propionic acid (PA) to that produced
as metabolite of bacterial overgrowth induced by clindamycin. Additionally, the protective effect of carnosine and carnitine as
two natural dietary supplements was assessed.
Single cell gel electrophoresis (comet assays) were performed on brain cortex and medulla after being removed
from the nine studied groups of hamsters, served as control, PAintoxicated, clindamycin- treated together with carnosine and
carnitine treated or protected groups.
The obtained data show a significant double strand breaks recorded as tail length; tail moment and % DNA damage
in PA and clindamycin-treated brain cortex and medulla compared to control-untreated hamsters. Moreover it proves the
neuroprotective effect of carnosine and carnitine. Receiver Operating Characteristics curve (ROC) analysis show satisfactory
values of sensitivity and specificity of the comet assay parameters.
Percentage DNA damage, tail length, and tail moment are feasible to be used as biomarkers of PA neurotoxicity
when given either orally or as metabolite of induced enteric bacterial overgrowth. Establishing biomarkers for these two
exposures is of importance to protect the health of children and could be helpful in controlling the prevalence of autism, a
disorder recently related to PA neurotoxicity
Amina ELgezeery has done her Ph.D. in Biochemical Human Genetics, Medical Research Institute Alexandria University, Egypt. She is an Associate Professor
of Biochemistry Department, King Saud University, Saudi Arabia. She was an Associate Professor at Human Genetics Department, Medical Research Institute
Alexandria University, Egypt. She has worked as an Ass. Prof. of Biochemistry at the Biochemistry Department, College of Science, King Saud University, Saudi
Arabia. She holds a membership of Scientific Committee of the Genetic Counseling Society
, Alexandria, Egypt.
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