alexa Comparison Of Serologic Tests For Epidemiological Diagnosis Of Bovine Babesiosis In México
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

7th International Veterinary Congress
September 04-05, 2017 | Paris, France

Julio V Figueroa, Irene M Castillo, Jose J Lira, Roberto O Castaneda, Antonio Cantu, Felix Mejia, Diego J Polanco, J Antonio Alvarez and Carmen Rojas
National Research Center for Veterinary Parasitology, Mexico
Posters & Accepted Abstracts: J Vet Sci Technol
DOI: 10.4172/2157-7579-C1-025
Abstract
The high economic impact caused by one of the most important tick-borne disease in Mexico, bovine babesiosis, has led to the search and development of effective techniques that facilitate its diagnosis and control. The objective of this study was to compare the serological indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (iELISA) for the identification of cattle exposed to Babesia spp. Negative and positive sera controls, sera from experimentally infected animals and sera from animals under field conditions were evaluated. For the iELISA, the recombinant proteins RAP- 1α (Babesia bigemina) and MSA-1 (B. bovis) were used as antigens. The technique iELISA demonstrated high sensitivity and diagnostic specificity rates using the RAP-1-α antigen. The sensitivity and diagnostic specificity rates for the indirect ELISA with the B. bigemina RAP-1-α recombinant antigen were 90% and 98.75%, respectively, whereas a sensitivity and diagnostic specificity of 73.33% and 81.25%, respectively, were obtained with the IFAT. The kappa index (κ) between the IFAT (in vitro culture-derived B. bigemina antigen) and iELISA (RAP-1-α) was 0.44. Although the serological tests do not have the capacity to discriminate cattle from three different geographic regions in Mexico that are exposed to either Babesia bovis and / or B. bigemina, it was concluded that the iELISA (RAP-1-α) is a tool with high sensitivity and diagnostic specificity, able to lead to a better knowledge on the epidemiology of bovine babesiosis, providing useful information for the design of control strategies and management of cattle babesiosis, one of the most important tick-borne disease in Mexico.
Biography

Julio V Figueroa is currently working as a researcher and head of the National Research Center for Veterinary Parasitology, INIFAP, in Jiutepec, Morelos, Mexico. He obtained his Veterinary Medicine Degree from The State of Mexico Autonomous University in Toluca, Mexico, and the MSc and PhD degrees in Veterinary Pathology and Microbiology at the University of Columbia-Missouri, in Columbia, MO, USA. He has conducted research on tick borne diseases of cattle during the past 30 years and has published over 70 research papers in peer reviewed international journals.

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version