Reach Us +1-504-608-2390
Comparison Of The Cytotoxic Effects Of Silver Sulfide Quantum Dot Coated With 2-mercaptopropionic Acid And Meso-2,3-dimercaptosuccinic Acid In V79 Cells | 21566
ISSN: 2161-0495

Journal of Clinical Toxicology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Comparison of the cytotoxic effects of silver sulfide quantum dot coated with 2-mercaptopropionic acid and meso-2,3-dimercaptosuccinic acid in V79 cells

3rd International Summit on Toxicology & Applied Pharmacology

Deniz Ozkan Vardar, Ibrahim Hocaoglu, Havva Funda Yagci Acar and Nurşen Başaran

ScientificTracks Abstracts: J Clin Toxicol

DOI: 10.4172/2161-0495.S1.012

Q uantum dots are semiconductor nanocrystals (~2?100 nm) with unique optical and electrical properties currently applied in biomedical imaging and electronics industries. One of the most valuable properties of QDs is their fluorescence spectrum, which renders them optimal fluorophores for biomedical imaging. The information about their effects in the cellular environment and their cytotoxic effects are still lacking. So the aim of this study was to assess and compare the in vitro cytotoxicity of silver sulfide quantum dots coated with 2-mercaptopropionic acid and Meso-2,3-dimercapto succinic acid. For this purpose Chinese Hamster Lung Fibroblast cell lines (V79) were treated with Ag2S-(2-mercaptopropionic acid) and Ag2S- (Meso-2,3-Dimercapto Succinic acid) quantum dots in the concentration range of 5-2000 μg/mL for 24 h. Cellular responses and their effects were characterized. The assays used are based on different modes of detection like MTT metabolism and neutral red uptake. Neutral red (3-amino-m-dimethylamino-2-methyl-phenazine hydrochloride) has been used previously for the identification of vital cells in cultures. This assay quantifies the number of viable, uninjured cells after their exposure to toxicants; it is based on the uptake and subsequent lysosomal accumulation of the supravital dye, neutral red. Another parameter used as the basis for colorimetric assays is the metabolic activity of viable cells. Tetrazolium salts are reduced only by metabolically active cells. Thus, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) can be reduced to a blue colored formazan. Quantum dots showed different cytotoxicity profile in a dose manner in both tests.
Deniz Ozkan Vardar has completed his bachelor?s and master degree at Gazi University. She is PhD student in Department of Nanotechnology and Nanomedicine, Hacettepe University. Her academic work is focused onnanotoxicology especially nanogenotoxicology. She has published 2 papers about genotoxicity. She has worked for more than 5 years in the Departments of Health Programs, Hitit