alexa Comprehensive Examination Of Intracellular Metabolite Of Pseudomonas Taiwanensis VLB120 Using Liquid Chromatographic Techniques Coupled With Mass Spectrometer Detection
ISSN: 2332-0737

Current Synthetic and Systems Biology
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3rd International Conference on Systems and Synthetic Biology
July 20-21, 2017 Munich, Germany

Gossa Garedew Wordofa
Technical University of Denmark, Denmark
Posters & Accepted Abstracts: Curr Synthetic Sys Biol
DOI: 10.4172/2332-0737-C1-009
Abstract
Absolute quantification of free intracellular metabolites is a meaningful tool in both pathway discovery and metabolic engineering efforts. Today, improved metabolomics tools make it possible to detect and quantify a wide range of metabolites providing useful information for pathway discovery and to improve the understanding of cellular systems and metabolism. However, most of these tools were designed for specific classes of metabolites and microorganisms; but in many studies sampling protocols are simply taken from the literature without validating them for the given conditions and the investigated organism, which can lead to highly biased results. Thus, sampling, quenching and extraction protocols need to be validated for each microorganism and metabolite of interest. Currently, we are investigating the metabolic state of Pseudomonas taiwanensis VLB120 to provide a useful reference dataset of absolute intracellular metabolite concentrations. We are also testing the suitability of different metabolomics tools with the objective of identifying a better sampling and quenching/extraction techniques for P. taiwanensis VLB120 metabolome study.
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