Construction of a recombinant vaccinia virus expressing E gene of Japanese encephalitis virus and immunogenicity in pigs
16th Euro Global Summit and Expo on Vaccines & Vaccination
June 19-21, 2017 Paris, France

Hui-jun Lu

Changchun Veterinary Institute of CAAS, China

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Japanese encephalitis virus (JEV) can infect humans and swine with a high incidence rate in Asia. Consequently, the development of a vaccine that can provide rapid protection with minimal risk is an important research goal. A recombinant vaccinia virus (rVVTK/E3L�?�??E) containing envelope protease coding regions of JEV Yunnan0901 was constructed in this study. With the Tiantan strain of vaccinia virus (E3L�?), an attenuated vaccine was constructed with TK gene deletion by homologous recombination. After screening and identification with a selectable marker, the recombinant vaccinia virus candidate vaccine was evaluated for its ability to induce humoral and cellular responses in pigs. The animals were vaccinated twice with a 21-day interval, and groups of animals were inoculated with the attenuated vaccine, vaccinia virus or phosphate buffered saline (PBS). All pigs vaccinated with rVVTK/E3L�?�??E developed specific anti-JEV antibodies and neutralizing antibodies. Splenocytes from pigs immunized with rVVTK/E3L�?�??E showed higher levels of T-lymphocyte proliferation, the greatest amounts of interferon-γ and interleukin (IL)-2, and moderate amounts of IL-4 and IL-10 in the presence of JEV. Vaccination with rVVTK/E3L�?�??E provided greater protection than that by the attenuated vaccine against JEV challenge. Moreover, pigs inoculated with the recombinant vaccine rVVTK/E3L�?�??E showed a lower virus load than those given the attenuated vaccine. Our findings indicated that rVVTK/E3L�?�??E might be an attractive candidate vaccine for preventing JEV infection.

Biography :

Email: huijun_lu@126.com