Construction of molecular breeding platform using population sequencing information in rice

3^rd International Conference on Genomics & Pharmacogenomics

September 21-23, 2015 San Antonio, USA

Longbiao Guo, Dali Zeng, Zhenyu Gao, Guangheng Zhang, Li Zhu, Jiang Hu and Qian Qian

China National Rice Research Institute, China

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

We re-sequenced a biparent genetic population including 132 core Recombinant Inbred Lines (RILs) of Liang-You-Pei- Jiu (LYP9), the widely cultivated super hybrid rice and constructed a high-resolution linkage map with 171,847 Single Nucleotide Polymorphism (SNP) markers. This is the first to have successfully assembled the genome sequence of the maternal cultivar PA64 of LYP9 and we have significantly improved the genome sequence of the paternal variety 93-11. Based on the highdensity physical map, we detected 43 yield-associated quantitative trait loci, of which 20 are unique. Also these genetic materials were used in whole genome transcription analysis. Using the IlluminaHiSeq 2000 technique and the 132 RILs, the transcript expression of eQTL in different tissues of heading stage was analyzed. Compared with the yield QTLs, some stable or differential eQTLs were found. In the leaves and seeds, there were 270 and 2791 F1 differential expresses, respectively, relative to their parents mainly involved in carbon metabolism and plant hormone signal transduction pathway. We also analyzed specific expression of allele genes and more than 70% of the transcripts of allelic gene expression trends to the male parent. Multiple genome modules were also identified to control the same trait or a few characteristics. The result has provided an ideal platform for dissecting yieldassociated loci, eQTL in super hybrid rice and molecular breeding.

Biography :

Email: guolongbiao@caas.cn