The experimental technique for the repair of bone lesions is based on the construction of tridimensional scaffolds colonized by
osteogenic cells prior to the implantation. The optimum material for scaffold construction should allow for the mechanical
rigidity until the formation of bone, and the resorption of scaffold thereafter. The material used for scaffold production have to be
"stem cell friendly"and moldable into three-dimensional shape.
Scaffolds were fabricated from polycaprolactone (PCL) or PCL plus 5% tricalcium phosphate (TCP) using Solid Form
Fabrication method with the lay-down pattern of 0
, which resulted in the honeycomb-like structure. Human or rat
adipose-derived stromal cells (ADSC) were isolated, phenotypically characterized and expanded in vitro. Plastic-adherent cells
(CD29+ CD44+,CD71+, CD90+, CD105+, SH3+, CD31-, CD34-, CD45-), capable of osteogenic differentiation in vitro, were
seeded in densities 5 x 10
/ml - 5 x 10
/ml into scaffolds and cultured in basic medium or medium supplemented with osteogenic
stimulators. After 2-6 weeks scaffolds were analyzed microscopically. The in vivo experiment on rats was performed with scaffolds
seeded with ADSC isolated from allogeneic and autologous rat donors and implanted in various degrees of proximity from large
The preliminary results confirm, that ADSC are able to colonize scaffolds (30% higher expansion rate in PCL + TCP than
in PCL alone) and differentiate into osteogenic cell lineage. In vivo implanted scaffolds do not produce any adverse reactions,
and integrate with surrounding connective tissue. Several aspects of scaffold construction, cell seeding and differentiation, and
in vivo implantation are discussed.
This work was financed by the European Regional Development Fund within the Innovative Economy
Operational Programme in the frame of BIO-IMPLANT project.
Zygmunt Pojda, M.D., Ph.D. is the chairman of Department of Cellular Engineering, M. Sklodowska-Curie Memorial Cancer Center and Institute
of Oncology, Warsaw, Poland. His former scientific interests were experimental hematology and radiobiology, currently he is involved in stem cell
research and regenerative medicine projects. He have published over 100 papers in renowned journals. He is the partner or coordinator of several
European Community research projects.
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