Journal of Bacteriology & Parasitology

Correlation between the number of ProAla repeats in the EmrA-homologue of Acinetobacter baumannii and resistance to Tobramycin, Netilmicin and Imipenem

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Alicja Nowak-Zaleska1, Roman Kot�?owski2, Agnieszka Mikucka3 and Eugenia Gospodarek3

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

The occurrence of multidrug resistant (MDR) strains of A. baumannii is a growing problem especially when concerning
patients in intensive care units. One of the causes of antibiotic resistance is the presence of transporter proteins responsible
for their removal. So far, for strains of the species A. baumannii, a system of transporting antibiotics composed of three
proteins, AdeA, AdeB and AdeC responsible for resistance to gentamicin, ofloxacin, cefotaxime, and tetracycline has been
described. Multiple alignment revealed a 39% similarity of CAP01997 protein of A. baumannii containing Pro-Ala repeats to
EmrA that is part of the EmrA/EmrB/TolC transportation system of E. coli responsible for mutlidrag resistance. We took tryin
to check whether there is a correlation between the number of Pro-Ala repeats in the EmrA homologue of A. baumannii and
the resistance to antibiotics. In this study 79 MDR A. baumannii strains isolated from patients were analyzed. Resistance to
antibiotics was determined on Mueller–Hinton agar plates using a Kirby–Bauer disk diffusion test. The number of CCTGCA
repeats encoding Pro-Ala repeats in EmrA-homologue of A. baumannii was determined using PCR method and capillary
electrophoresis. The results of this research showed significant correlation between resistance to tobramycin and netilmicin,
susceptibility to imipenem and the numbers of repeated Pro-Ala sequences in the CAP01997 protein, a homologue of EmrA.
Based on presented results (p<0.0001) we can conclude that EmrA-homologue of A. baumannii confers resistance to
Imipenem and Netilmicin depending on the presence of 7 and 5 Pro-Ala repeats in CAP01997 protein, respectively.

Biography :

Alicja Nowak-Zaleska, PhD, dissertation in the field of genetic differentiation of the genus Acinetobacter, a microbiologist, an assistant in the Department of Biology,
Ecology and Sport Medicene. Roman Kot�?owski, PhD, chemistry, an assistant in the Department of Molecular Biotechnology and Microbiology. Agnieszka Mikucka,
MD, microbiologist, deputy head of Dept. of Microbiology, Nicolaus Copernicus University in Toru�?, Collegium Medicum of L. Rydygier in Bydgoszcz. Eugenia
Gospodarek, MD, microbiology, prof. NCU in Toru�?, head of Dept. of of Microbiology Nicolaus Copernicus University in Toru�?, Collegium Medicum of L. Rydygier
in Bydgoszcz.