Pharmaceutica Analytica Acta

Cross linked chitosan based gel as self modifying bioadhesive film for enhanced wound healing

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Pandey Suneel and Patil Arun

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

The present study intended to formulate a cross linked self modifying bioadhesive film forming chitosan based hydrogel for heighten wound healing activity of Ciprofloxacin hydrochloride. Chitosan hydrogels have been prepared in 1% lactic acid and in situ cross linking through various metal viz. zinc chloride, copper sulphate, calcium chloride, and aluminium chloride (cytotoxic effects of cross-linking cation and chitosan on skin cells were established before use). Application of polyvalent cations as cross linking to a chitosan hydrogel resulted in an indissoluble, elastic, gentle rubbery structure within 90 seconds and further converted into a film within 8-10 min onto the skin. Transdermal epidermal water loss through formed film and mechanical properties of cross linked gel as well as films were also determined: In order to evaluate its wound healing effect, full-thickness skin incisions were created on dorsal surface of the rat below the cervical region. A cross linked chitosan hydrogel was applied onto the wound. Cross-linking agents, released from chitosan dressings during use could interact with the anti-microbial agent and significantly affect the therapeutic outcome. The macroscopic appearance indicated that the wound healing occurred by 6th day for the treated groups whereas it was noticed only on the 10th day in the control animals. Complete healing of the wound in dressing applied groups took only 14 and 18 days in case of marketed formulation, whereas it took 24 days for the control groups. On the 4th day, the wound contraction of control was 13% whereas 21% for marketed formulation and 35% closure were observed for film forming chitosan based hydrogel. Due to its ability to accelerate wound contraction and healing, situ film forming chitosan hydrogels may become accepted as an occlusive dressing for wound management.