alexa Culture Dependent And Independent Analyses Of Throat Swabs Reveal Role For Bacterial Pathogens Other Than Group A Streptococcus In Pharyngitis
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on Clinical Microbiology & Microbial Genomics
September 16-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Shunmugiah Karutha Pandian
Accepted Abstracts: J Microb Biochem Technol
DOI: 10.4172/1948-5948.S1.011
Abstract
Till date, group A Streptococcus (GAS) are believed to be the major causative agent for bacterial pharyngitis. The undisclosed co-existence and possible contribution of other bacteria in causing pharyngitis is to be highly focused and explored in order to gain greater understanding to improve our knowledge in prescribing appropriate methods and measures for its prevention. Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) analysis are nucleic acid based techniques which were used in the present study to explore the prevalence of bacterial communities other than GAS. Throat swabs from pharyngitis patients and healthy subjects were collected and subjected to metagenomic DNA extraction and analysed using T-RFLP and DGGE. The results from the culture independent techniques revealed an unexpected diversity of causative pathogens like Neisseria sp., Haemophilus influnzae, Klebsiella pneumonia, Staphylococcus aureus etc from throat swabs of pharyngitis patients compared to that of healthy subjects. Furthermore, the results clearly depicted the higher prevalence of GAS in throats of pharyngitis cases than that of healthy individuals. In addition, the band intensity analysis of DGGE showed a rich occurrence of S. aureus in the pharyngitis throats which made us curious to study its prevalence in pharyngitis cases through culture-dependent analysis. The molecular epidemiology (culture-dependent analysis) of S. aureus in throat swabs (n=265) of pharyngitis patients revealed that 165 swabs as positive, among which 63 were found to be methicillin-resistant and the remaining as methicillin-susceptible S. aureus . An interesting result was that amongst the 63 throat swabs positive for MRSA, 33 were found to co-exist with GAS and that implies that the remaining 30 pharyngitis cases could be due to the infection of active MRSA. Further, 26 MRSA isolates were selected for the molecular characterization through multilocus sequence typing and eBURST analysis, which resulted in 12 different sequence types (STs), including a novel ST2129 and 5 different clonal clusters (CCs) respectively. The antimicrobial susceptibility patterns of the recovered MRSA isolates showed high level of multidrug- resistance, including 10 MRSA isolates intermediate to vancomycin. From the results of the culture dependent and independent analyses, we hypothesize that S. aureus , particularly MRSA could play a possible role in pharyngitis infection on its own or in association with GAS.
Biography
Shunmugiah Karutha Pandian is the Head of the Department of Biotechnology and the Dean of Research at Alagappa University, Karaikudi, Tamil Nadu, India. His research group is actively working on studying the biofilm formation and gene regulation of various human bacterial pathogens, and characterizing novel anti-quorum sensing and antibiofilm compounds against human and aquaculture pathogens from marine resources. He has published more than 100 research papers in SCI journals including 50 papers in the field of clinical microbiology
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]line.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords