Journal of Vaccines & Vaccination

Cyclooxygenase and 5-Lipoxygenase inhibitory dammarane triterpenoid 1 as anti-inflammatory and anticancer agent

8^th Indo Global summit and Expo on Vaccines, Therapeutics & Healthcare

November 02-04, 2015 HICC, Hyderabad, India

Nagendra Sastry Yarla

GITAM University, India

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Phospholipase A2 (PLA2), Cyclooxygenase (COX) and 5-Lipoxygenase (5-LOX) are arachidonic acid metabolizing enzymes and their inhibitors have been developed as therapeutic molecules for cancer and inflammation related disorders. In the present study, PLA2, COX 1&2 and 5-LOX inhibitory studies of Borassus flabellifer seed coat extract were carried out and substantial 5-LOX inhibitory activity was found. Dammaranetriterpenoid 1 (Dammara-20, 23-diene-3, 25-diol) was isolated according to 5-LOX activity guided isolation, and screened for COX (1 & 2) inhibitory activities. Dammarane triterpenoid 1 inhibited carrageenan-induced rat paw edema and TNF-α secretion levels in lipopolysaccharide (LPS)-induced THP-1 human monocytes. Anticancer activity studies demonstrated the anti-proliferative effect of dammarane triterpenoid 1 on various cancer cell lines including MIA PaCa-2 pancreatic, DU145 prostate, HL-60 leukemia and Caco-2 colon cancers. Dammarane triterpenoid 1 showed good anti-proliferative activity on MIA PaCa-2 pancreatic cancer cell line with IC50 of 12.36±0.33 μM, among other tested cell lines. Apoptosis inducing activity of dammarane triterpenoid 1 was confirmed based on increased sub-G0 phase cell population in cell cycle analysis, loss of mitochondrion membrane potential, elevated levels of cytochrome c, nuclear morphological changes and DNA fragmentation in MIA PaCa-2 pancreatic cancer cells. Therefore, dammarane triterpenoid skeleton may raise the hope of developing novel anti-inflammatory and anticancer drugs in the future.

Biography :

Nagendra Sastry Yarla has completed his Secondary School Education from Board of Secondary Education, Andhra Pradesh in 2003 with 80 percentage and studied his Higher Secondary Education from Board of Intermediate Education, Andhra Pradesh in 2005 with 60 percentage and got into his BSc in Biotechnology, Biochemistry and Microbiology from Andhra University in 2008 with 68 percentage and achieved his Masters (MSc) in Biochemistry from GITAM University, Visakhapatnam in 2011 with 8.68 CGPA. He is currently pursuing his PhD in Gitam University, Visakhapatnam, India. He has published 65 research articles, filed 2 patents and is the author of 4 books.

Email: sastryyn@gmail.com