alexa Cytoprotective Effects Of Antioxidants On Articaine-Induced Strocyte Toxicity | 70936
ISSN: 2155-9562

Journal of Neurology & Neurophysiology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

14th World Congress on Neurology and Neurological Disorders

Shilpa Jhol, MS; Richard White, PhD, FAHA; Harry Komiskey, PhD
Georgia-Philadelphia College of Osteopathic Medicine, USA
Posters & Accepted Abstracts: J Neurol Neurophysiol
DOI: 10.4172/2155-9562-C1-056
Abstract
Introduction: As the push to reduce clinic wait times and healthcare spending costs are on the rise, administration of local anesthesia is becoming a popular option in a vast majority of surgical procedures. Anesthetic drugs are thought to mainly target neurons in the brain by suppressing synaptic activity. However, the direct effects of local anesthetics on astrocyte signaling remain largely unknown. Antioxidants such as Nacetylcysteine (NAC) and ubiquinol, may reduce astrocyte reactive oxidative species (ROS) and improve mitochondrial respiration. In the current study, we propose to investigate the cytoprotective effects of these antioxidants on astrocytes cotreated with articaine and understand articaine’s direct effect on astrocyte Kir4.1 channels. Methodolgy: Embryonic rat hippocampal tissue (E18) was obtained and cultures were grown until they reached 80% confluence. Cells were treated with NAC (30uM, 100uM, 300uM) or ubiquinol (30nM, 100nM, 300nM) in a dose-dependent manner for 24 hours and co-treated with articaine (10uM) for an additional 24 hours. Cellular potassium currents were also measured directly via whole-cell patch-clamp technique at a holding potential of -50 mV. Mitochondrial biogenesis was examined using immunofluorescence. Conclusions & significance: Both NAC and ubiquinol increased cell viability significantly and reduced the amount of glial fibrillary protein (GFAP) released from rat embryonic astrocytes. Whole-cell readings revealed an inward-rectifying current with a reversal potential of -40mV- -50mV. Addition of barium or articaine attenuated inward-potassium currents. Cells treated with NAC and ubiquinol also showed an upregulation in mitochondrial biogenesis. A novel cellular transduction mechanism may contribute to articaine’s toxic effects on astrocytes and thereby decrease astrocyteneuron signaling. Comparative studies need to be performed to understand articaine’s influence on astrocyte-neuron signaling.
Biography

Shilpa Jhol has a Master of Science degree from the Georgia-Philadelphia College of Osteopathic Medicine. Under the direction of her advisor, Dr. Komiskey, Shilpa’s current thesis research focuses on how antioxidants and local anesthetics affect astrocyte behavior. Shilpa’s passion to understand astrotoxicity stems from her interest in learning about neurological diseases. Shilpa attained her bachelors of science in Neuroscience and Behavioral Biology at Emory University in 2015 and is currently waiting on admittance to medical school.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

kactakapaniyor.com

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

Taktube

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

sikiş

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

https://www.gaziantepescort.info

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

putlockers

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

seks

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version