alexa Cytotoxic Effects Of Bromelain And N-acetylcysteine On The Gastric Carcinoma Cell Line KATO-III
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd World Congress on Cancer Science & Therapy
October 21-23, 2013 DoubleTree by Hilton Hotel San Francisco Airport, CA, USA

Afshin Amini, Anahid Ehteda, Samar Masoumi Moghaddam and David Lawson Morris
Posters: J Cancer Sci Ther
DOI: 10.4172/1948-5956.S1.029
Abstract
Background: Bromelain is the extract of pineapple stem primarily comprised of sulfhydryl containing proteolytic enzymes. N-acetylcysteine (NAC) is also a sulfhydryl-containing compound found in allium plants, asparagus, and red pepper. Biological effects of bromelain and NAC have been studied in some pathologic conditions including cancer. In the present study, we investigated the cytotoxic effects of bromelain and/or NAC on the gastric carcinoma cell line KATO-III in vitro. Methods: KATO-III gastric carcinoma cells were treated with a range of concentrations of bromelain and/or NAC. The effect of the single agent or combination therapy on the growth and proliferation of the cancer cells was determined after 72 hours of treatment using sulforhodamine B assay. A similar method was employed to assess any possible synergism when the treatment was combined with chemotherapy. Results: We observed that bromelain, at the concentration of 200 μg/ml, and NAC, at the concentration of 25 mM, significantly inhibited the cell proliferation (p-values of <0.0001 and 0.0095, respectively). When combined together, synergistic effects of the combination therapy was evident. In addition, we examined if the treatment was capable of potentiating the cytotoxic effects of chemotherapy. Cytotoxicity of single agent cisplatin, paclitaxel, 5-fluorouracil and vincristine was compared against that of their combination with bromelain/NAC. Our results indicated a higher potency of each drug when used in combination with bromelain/NAC. Conclusion: In the present study, we showed that the utility of bromelain and N-acetylcysteine, as single agent or in combination, yields cytotoxic effects in the gastric carcinoma cell line KATO-III and potentiates chemotherapy.
Biography
Afshin Amini completed his doctoral degree in Medicine in 1997. Being interested in cancer research, he was granted a competitive international postgraduate scholarship by the University of New South Wales (Sydney, Australia) in 2012. As a member of Professor Morris?s research team at St George Hospital, Afshin is currently involved in some key projects aimed to develop novel approaches to cancer therapy.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords