alexa Depth Filtration As A Method Of Primary Recovery Of Human Cells For Therapy | 22378
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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3rd International Conference and Exhibition on Cell & Gene Therapy

Chris Longster, Ivan Wall, Mike Hoare and Peter Levison
Accepted Abstracts: J Stem Cell Res Ther
DOI: 10.4172/2157-7633.S1.009
Abstract
Human cell therapies will offer an exciting alternative to biopharmaceuticals provided the challenges associated with whole cell bioprocessing are addressed. One such challenge is the primary recovery and washing of harvested cells prior to formulation and patient administration. The recovery of adherent cells is often carried out through multi stage batch centrifugation; a manual process with inherent risk of contamination. Also the hydrodynamic stresses experienced during transfer stages such as cell resuspension can cause cellular damage as well as loss of biopotency and/or viability. An ultra scaledown approach to the development of dead-end filtration for the low stress recovery of cells is presented. The device allows the study of cell loading, washing and recovery via a backflush step. An experimental design will be presented of filtration bioprocessing studies using small quantities of cells available at early stages of process development. Preliminary results will show the impact of how the method of loading and the subsequent backflushing step affects the recovery of viable cells.
Biography
Chris Longster is a final year EngD student at University College London. Having completed a Bachelor?s degree in Biomedical Science he moved to UCL to complete Masters in Biochemical Engineering before beginning his EngD in 2010. His current research project is developing a USD filtration device for the preparation of adherent cells for therapy is a collaborative between UCL and Pall Life Sciences.
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