alexa Derivation Of Mesenchymal Stem Cells From Umbilical Cord By Comparing Two Enzyme Digestion Methods: Differentiation Of Osteogenic Cells From UC MSCS | 71662
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

8th International Conference on Tissue Science and Regenerative Medicine

Fathima Zahara Mansoor, H Goonasekara and V Dissanayake
University of Colombo, Sri Lanka
Posters & Accepted Abstracts: J Tissue Sci Eng
DOI: 10.4172/2157-7552-C1-037
Abstract
Aim: The aim of the study is to derive MSCs from the human UC Wharton’s Jelly (WJ) and osteogenic differentiation. Method: Umbilical cord (UC) is a readily available without ethical constraints, showing high proliferation rate and osteogenic potential. Following obtaining ethical approval, five UCs from healthy mothers undergoing elective caesarian sections were collected, cleaned with phosphate buffered saline, removed blood vessels, digested WJ in 0.5% collagenase 2-3 hours/0.2% collagenase overnight and cultured in DMEM supplemented with 10% FBS, 1% L-glutamine and 1% Pen Strep at 37 oC in 5% CO2. Cells are passaged at 70% confluency. At fourth passage (P4), osteogenic differentiation medium was added following incubation. Culture maintained for 21 days and cells were stained with 2% Alizarin red and von Kossa stains. MSCs were determined and characterized using Trypan blue test, flow cytometry, RT-PCR and karyotypic analysis. Findings: Cells were positive for CD90, CD73 and CD105 and negative for CD34 and CD45 markers expressing Oct-4 and G6PD genes. Karyotypes depicted were normal. Alizarin red stain gave bright orange red and von Kossa stain gave black brown deposits demonstrating the presence of extracellular calcium deposits. Conclusion & Significance: UC-MSCS serves as a suitable source for osteogenic regeneration. Gene expression demonstrated the embryonic origin of the MSCs which maintained genomic stability up to P4 stage. So this initiative stem cell research in Sri Lanka improves the therapeutic potential in bone defects and opens up new perspectives for bone tissue engineering.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7