alexa Dermal Fibroblasts: An Alternative Source For Bone Regeneration | 17435
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th World Congress on Cell Science & Stem Cell Research

M Barba, C Cicione, G Di Taranto, M Baranzini, G Logroscino, C Bernardini, F Michetti and W Lattanzi
Posters: J Cell Sci Ther
DOI: 10.4172/2157-7013.S1.029
Abstract
T he research on cell-based strategies for bone regenerative applications is aimed at providing osteoprogenitors, which should be readily available and able to form a functional bone matrix in vivo. Bone marrow-derived mesenchymal stromal cells are mostly used for this purpose, though the small number of cells isolated and the risk of donor site morbidity limit their use. Osteogenic progenitor cells derived from dermal tissue may provide a more abundant and easily expandable source of cells. The aim of this study was to assess the spontaneous osteogenic potential of dermal fibroblasts, through the comparative analysis of cell behaviour and matrix production of dermal fibroblasts (SDF) and MSCs. SDF were isolated in primary culture from skin biopsies and cultured both in growth medium (GM) and in osteogenic medium (OM) for 10 days after reaching confluence. The expression of osteo-specific genes (RUNX2, OC, OSX, BMP2, OPN) was analyzed through qPCR, and matrix deposition was assessed by Alizarin Red staining. The results revealed that osteogenesis was efficiently induced by OM in MSC, increasing the expression of osteo-specific genes and enhancing mineralised matrix deposition. Conversely, the osteogenic potential of SDF grown in GM was greater than that induced by OM, as revealed by athe significantly higher expression of osteo-specific genes. Moreover, after 10 days of culture, SDF aggregated in spheroid structures that were analyzed morphologically and confirmed the presence of a mineralized matrix. These data suggested that undifferentiated SDF are spontaneously prone to commit towards an osteogenic lineage and could be thus suitable for the development of cell-based treatment for bone regeneration
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7