alexa Detection And Identification Of Microorganisms By Nanoparticles Based Biosensors
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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4th International Conference on Nanotek & Expo
December 01-03, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Sam F Y Li and Haiyan Li
ScientificTracks Abstracts: J Nanomed Nanotechnol
DOI: 10.4172/2157-7439.S1.017
When self-assembled monolayer (SAM) by 11-Mercaptoundecanoic acid (MUA) and AuNPs were used to modify the sensor surface and to amplify the signals, quartz microbalance (QCM) immunosensors showed good detection sensitivity, i.e., detection limit as low as 102 CFU/mL for E. coli in less than 30 min could be achieved. Though the modification process on the sensor surface still involved several steps, the immobilization process could be readily replaced by easy regeneration of antibodies immobilized sensor surface. Fe2O3/Au core/shell nanoparticles were fabricated to improve surface functionality and reduce surface oxidizationof iron oxide. By using SAMs with MUA and functionalized Fe2O3/Au nanoparticles, an improved QCM biosensor with detection limit of 102 CFU/mL for bacteria detection could be obtained. The detection sensitivity of QCM biosensor with Fe2O3/Au nanoparticles can potentially be improved after further optimizing of Fe2O3/Au nanoparticles.
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