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Detection of Bt protein metabolites presence in insect and their predator transferred through GM rice using ELISA technique
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Metabolomics:Open Access

ISSN: 2153-0769

Open Access

Detection of Bt protein metabolites presence in insect and their predator transferred through GM rice using ELISA technique


5th International Conference and Exhibition on Metabolomics

May 16-18, 2016 Osaka, Japan

Zunnu Raen Akhtar

University of Agriculture Faisalabad, Pakistan
Zhejiang University, China

Scientific Tracks Abstracts: Metabolomics

Abstract :

Transgenic crops were developed to show resistance against the target chewing insects in transgenic crops. Genetically modified rice was also developed to show the resistance to its major insect pests such as Lepidopteran in paddy rice. However some nontarget effects were found from genetically modified crops. Stenchaetothrips biformis, the most important rice seedling insect pests and one of nontarget insect species of Bt rice. In this experiment ELISA was performed for three Bt rice lines including H2A, H1C, E1 (expressing the Cry2A, Cry1C and Cry1Ab/Vip3H protein respectively) and their non-Bt correspondent parental cultivars HCK and Xiushui 110 under laboratory conditions. For thrips and predator Orius sp., ELISA was performed to observe the uptake of Bt toxin from transgenic plants to insects and ultimately upto third tropic level. Although sucking insects have no specific site of action of Bt toxin in digestive system. A leaf dip bioassay was carried using the leaf from control cultivar. Different concentration of Cry2A, Cry1C and Cry1Ab proteins in bioassay to observe the effects on mortality of S. biformis at different levels was also conducted. Higher levels of Bt protein were detected in case of H2A (Cry2A) rice plants and higher concentration was observed in case of larvae as well as adult thrips. While higher concentration of Bt protein in case of H1C (Cry1Ac) was also observed. Normal levels of Bt protein were detected in case of E1 (Cry1Ab) concentration in Bt rice plants, larvae as well as adult thrips. While no Bt protein was detected in case of control cultivars in all tested control cultivars. No Bt protein was detected in predator Orius sp.

Biography :

Zunnu Raen Akhtar has completed his PhD at the age of 30 years from Zhejiang University, China and postdoctoral studies from Zhejiang University School of Biotechnology. He is the Assistant Professor of Biotechnology a well renowned ranked 2nd Pakistani University. He has published more than 10 papers in reputed journals. He is an expert of Biotechnology in his country and works on preparation of GM crops and their proteomic and metabolomic analysis.

Email: zunnuraen@gmail.com

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Citations: 895

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