alexa Detection Of Ochratoxine A Using Square Wave Voltammetry On BDD Film Microcells
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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In Association with

3rd International Conference and Exhibition on Biosensors & Bioelectronics
August 11-13, 2014 Hilton San Antonio Airport, San Antonio, USA

A Chrouda, A Sbartai, A Maaref and N Jaffrezic-Renault
Accepted Abstracts: J Biosens Bioelectron
DOI: 10.4172/2155-6210.S1.022
In this work we used a planar electrochemical microcells micromachined in a microcrystalline BDD thin layer using a femtosecond laser. They were designed for fitting in a flow-through cell. This microcell was first derivatized by electrochemical reduction of in situ generated 4-carboxymethylaniline diazoniumsalt (4-CMA) in acidic aqueous solution and yielded stable monolayer. Horseradishperoxidase (HRP) was then immobilized making use of the carbodiimide chemistry, which offers a higher rapidity and simplicity in the manufacturing process of biosensors for OTA determination. The square wave voltametric oxidation current registered has been successfully related to the concentration of OTA in solution from different samples. Under the optimum conditions of the experimental variables, precision in terms of reproducibility and repeatability has been calculated in the concentration range 1 pg.mL-1 to 25 ng.mL-1, with a detection limit of 0.001 ng.mL-1. A relative standard deviation for the slopes of 10% (n=4) was obtained for reproducibility. In the case of repeatability, the biosensor retained a 30% of the initial sensitivity after the third calibration.
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