Determination Of Efficiency For Enzymatic Delignification Of Lignocellulosics Using Laccase | 92387
Journal of Fundamentals of Renewable Energy and Applications
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Biological processes are becoming more competitive and gaining increased attention worldwide due to sustainability and
eco-friendly nature. Biocatalyst, such as enzymes produced from microorganisms act as an effective green catalyst for
biomass deconstruction. Laccase (oxidoreductase, EC 22.214.171.124) is a multicopper phenol oxidase enzyme that oxidizes electronrich
phenolic and non-phenolic substrates. Lignocellulosics such as Saccharum spontaneum (Kans grass), contains huge amount
of carbohydrates in its cell wall and to make this enormous amount more accessible for hydrolysis and to be used further in
fermentation, degradation of lignin through appropriate pretreatment process is an essential prerequisite of the complete biofuel
production process. In the present work, laccase obtained from Lentinus sp. has been used for biomass deconstruction. The
process was optimized through response surface methodology (RSM) based on central composite design (CCD) to investigate
the effects of the different process parameters on biomass pretreatment. The maximum delignification obtained was 81.67%
at 6 h of incubation time upon monitoring the initial lignin content of 17.46 %. Highest reducing sugar yield from enzymepretreated
Kans grass was 500.30 mg g-1 substrate after 5.30 h of incubation time at a low cellulase loading. SEM (Scanning
electron microscopy) analysis indicated changes in the surface characteristics, whereas FT-IR (Fourier transform infrared
spectroscopy) shows that the pretreatment condition does not pose any major changes in the chemical nature of cellulose and
hemicellulose. This work contributes towards the emergence of greener biomass pretreatment and utilization strategy.
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Rajiv Chandra Rajak is currently pursuing his PhD from Indian Institute of Technology Kharagpur, India. He is working in the area of biomass deconstruction using biological catalsyt and its role in reducing sugar production.