alexa Determination Of Ochratoxin A In Food Samples By Liquid Chromatography/electrospray Ionization Triple Quadrupole-MS-MS Spectrometry
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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4th World Congress on Chromatography
August 07-09, 2017 | Rome, Italy

Deming Song
Food and Drug Administration Northeast Region Laboratory, USA
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-032
Abstract
Ochratoxin A (OTA) is the most important compound in a group of structurally related mycotoxins. It is a potent carcinogen in rats and possibly associated with human kidney diseases in certain countries. OTA is a moderately stable molecule that will survive to some extent most food processing (e.g. boiling, baking, roasting or fermentation) and may thus occur in consumer products. Therefore, food chemistry branch, Northeast Regional Laboratory of FDA is monitoring OTA in a number of food products. We have established a method by HPLC-ESI-MS-MS for the analysis of OTA. By engaging SRM (selective reaction monitor) mode mass spectrometry for four OTA most distinguish and strong ions m/z 404, 386, 358 and 239, not only has the specific identification power against the method by HPLC-fluorescence detector, but also this method can reach the sensitivity of 1 parts per billion concentration (ppb) with injection volume 8 μl equivalent to 8 pg on column for quantification analysis of OTA.
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