alexa Development Of A Multi-epitope Antigen As A Serodiagnosis Marker Of Toxoplasma Gondii Infection | 67610
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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JOINT EVENT ON 15th International PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY CONFERENCE & 10th Annual MEDICAL MICROBIOLOGY SUMMIT & EXPO
June 21-23, 2017 London, UK

Abbas Alibakhshi, Mojgan Bandehpour, Zarin Sharifnia and Bahram Kazemi
Beheshti University of Medical Sciences, Iran
Posters & Accepted Abstracts: J Med Microb Diagn
DOI: 10.4172/2161-0703-C1-006
Abstract
Due to the high prevalence of Toxoplasma gondii infection has been attracting a lot of attention to its diagnosis and treatment. The use of pure antigens although in some show high sensitivity and specificity, however, the challenges such as cross-reactivity still remain as diagnostic difficulties. In this study, three surface antigens (SAG) of T. gondii were employed for designing a multi-epitope antigen. The antigen was then expressed using E. coli BL21 (DE3) cells and purified by affinity chromatography. ELISA analysis was used for evaluation of acute toxoplasmosis. The results showed a sensitivity of 72.6% and a specificity of 90.3% for recombinant ELISA.
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