alexa Development Of A SYBR Green Real-time PCR Standard For Porcine Parvovirus (PPV) And Screening Of Porcine Tissue DNA Samples | OMICS International
ISSN: 2161-0517

Virology & Mycology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

9th International Virology Congress and Expo
March 13-14, 2017 London, UK

Laxmi Pandey
St. Aloysius College, India
Posters & Accepted Abstracts: Virol Mycol
DOI: 10.4172/2161-0517.C1.018
Abstract
Swine viral diseases are of great concern as they hamper the economy of the swine industry. One of such major disease problem is porcine parvovirus (PPV) infection and is the major cause of reproductive failures in swine. Porcine parvovirus is an autonomously replicating parvovirus, belongs to genus parvovirus from the Parvoviridae family. Qualitative real time PCR is a method to rapidly and precisely quantify gene activity by detecting RNA level of gene of interest. In the present study we have developed an assay based on real time PCR for the screening of porcine parvovirus in tissue DNA samples. A recombinant plasmid carrying a fragment of VP2 gene was linearized by digesting with Nco I. Serial log 10 dilution of linearized plasmid was prepared and standard curve was generated by using this as the template. A total of 102 DNA samples were screened through real time and conventional PCR, 14 in real time and 4 samples in conventional PCR came positive. Real time PCR found to be 105 times more sensitive than the conventional PCR in PPV negative pig genomic spiking. So we can say that real time PCR though costlier but at the same time it is more sensitive and accurate technique over the conventional PCR.
Biography

Email: lkpandey16@gmail.com

image PDF   |   image HTML
 

Relevant Topics

OMICS International Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version