Development Of Edible Vaccine Against Anthrax By Using Edema Factor Gene Of Bacillus Anthracis | 11287
Journal of Clinical & Cellular Immunology
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Anthrax is a epizootic disease mainly affecting cattle, caused by the spore-forming bacterium
. It produces
three toxin components, protective antigen (PA), lethal factor (LF) and edema factor (EF). Lethal toxin (LF+PA) and edema
toxin (EF+PA) are known to cause lethality in anthrax infected animal models. The recent bioterrorism attack and the revival
of the disease enforce an urgent need for effective prophylactic measures and therapeutic formulations to combat the disease.
The currently used PA based anthrax vaccine is associated with several side effects due to its potential reactogenecity and also
requires protracted and complicated dosages schedule. Since the expression of hepatitis B surface antigen in model plant tobacco,
a number of antigenic proteins have been expressed in different plant systems. These plant based vaccines are especially attractive
as plants are free of human or animal diseases, reducing screening and purification costs. Also, they offer a palatable oral delivery
system that stimulates both systemic and mucosal immune response. Attempts are being made to develop improved anthrax
vaccines using antigens other then PA. Several immunization studies have proved that LF and EF alone, produce substantial
amount of antibodies and in combination with PA, strengthen PA mediated immunoprotection. Studies have also highlighted the
need of using mutants of anthrax toxin components in vaccine preparations. We have successfully cloned normal and mutated
EF gene in a plant expression vector and Indian mustard plant
was transformed via
transformation. Molecular and immunological studies have shown the stable integration and expression of EF gene in the plants
Prerna Chaudhary, has completed her Ph.D from school of life sciences, Jawaharlal Nehru University, New Delhi.
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