The aim of this study was to fabricate different structural fibrinogen (Fbg) microfibers (MFs) and microtubes (MTs) using
gel solvent extraction method with silicon tube as template. To improve the biocompatibility and bioactivity, surfaces of
both MFs and MTs were enriched with immobilized fibronectin (FN), using water soluble EDC for cross-linking. The ultrafine
structures and outer morphology of Fbg-MFs and Fbg-MTs were characterized with scanning electron microscopy, revealing
highly rough outer morphology. Cross-linking between FN and Fbg was characterized by FT-IR and fluorophore-conjugated
goat anti-mouse immunoglobulin G by fluorescence microscopy, presenting uniform distribution of FN throughout the Fbg-
MFs and Fbg-MTs. The proteolytic degradation of FN/Fbg-MFs and FN/Fbg-MTs could be controlled upto 8 weeks
plasmin, confirming its biodegradable properties. The biocompatibility and cell proliferation of the Fbg-MFs and Fbg-MTs were
assessed by measuring ATP activity (CellTiter-Glo Luminescent Cell Viability assay) in C2C12 fibroblast cells. Cell attachments
and proliferations on these scaffolds were further examined using fluorescence and SEM microscopic images. In summary, the
experimental results suggested that both Fbg-MFs and Fbg-MTs were biocompatible, and FN immobilized surface demonstrated
the improved cell adhesion, proliferation and long term degradation. Lastly, this study revealed that FN could be easily grafted
onto Fbg surfaces, and it could be a potential substratum for growth factors or drug release. Especially, in wound healing and
vascular tissue engineering, the developed Fbg scaffolds could promote and orient cell adhesion and proliferation.
Thanavel Rajangam completed his B.Pharmacy and M.Pharmacy from The Dr,M.G.R Medical University (India) and currently doing his 3
Department of Bionanotechnology, Gachon University (S. Korea), under the guidance of Dr. Seong Soo A.An.
Seong Soo A.An, Ph. D., working as an Associate professor in the department Bionano Technology at Gachon University, South Korea. He received
his PhD from Carnegie Mellon University in the department of Biological Science: Biochemistry/Biophisics. His main area of research is focuses on
Tissue Engineering, Neurodegenerative disease, Haemostasis and Nanomaterials.
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