Pharmaceutica Analytica Acta

Development of innovative resveratrol-loaded solid lipid nanoparticles using natural Theobroma grandiflorum seed butter

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Pedro Paulo Soldati1, Hudson Caetano Polonini1, Frederico Pittella da Silva1,2, Maria das Gra as Afonso Miranda Chaves1 and Nadia Rezende Barbosa Raposo1

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Solid lipid nanoparticles (SLNs) have been attracting increasingly attention as efficient drug carriers for dermal application. The use of natural lipids (such as butters) appears very promising, but they are still a challenge to overcome. Therefore, the aim of this work was to develop new resveratrol-loaded SLNs using natural Theobroma grandiflorum seed butter (R-SLN) to enable further use in topical application. For this, the T. grandiflorum seed butter chemical composition was analyzed by GC, presenting oleic, stearic and arachidic acid as the main constituents (41.80%, 34.40% and 10.90%, respectively), which are already known as skin penetration enhancers. The R-SLN was prepared by the high shear homogenization method. The T. grandiflorum seed butter and resveratrol (0, 2 and 10 mg) were used as the lipid phase and Pluronic F127 and ultrapure water as the aqueous phase. Both phases were heated to 55�C, separately, and the molten lipid was then dispersed in the aqueous phase by high-speed stirring in an Ultra-Turrax for 1 min at 12,000 rpm, followed by 10 min of 35% intensity sonication. The nanodispersions were cooled at room temperature. Dynamic light scattering measurements revealed a hydrodynamic diameter of 140-147 nm, narrow particle size distribution (0.20-0.34), and zeta potential between -26.36 and -36.27 mV. These characteristics remained unchanged for 30 days, with no statistically significant differences (p>0.05), suggesting good colloidal stability. The drug encapsulation efficiency of R-SLN 2 mg and R-SLN 10 mg were 54.35% and 73.27%, respectively. The SLNs prepared in this study suggest a promising use in topical delivery, since seed butter presents constituents suitable for this application and the nanoparticles exhibit good physicochemical characteristics.