alexa Development Of PCR-ELISA For Specific And Sensitive Detection Of Mycoplasma Genitalium
ISSN: 2327-5073

Clinical Microbiology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

7th Euro Global Summit on Clinical Microbiology and Mycotoxins
February 27-28, 2017 Amsterdam, Netherlands

Arghavan Mokari Golkhatmi, Hadi Farsiani, Lena Goshayeshi, Hoda Radmanesh and Saeid Amel Jamehdar
Imam Reza Hospital-Mashhad University of Medical Sciences, Iran
Antimicrobial Resistance Research Center-Mashhad University of Medical Sciences, Iran
Posters & Accepted Abstracts: Clin Microbiol
DOI: 10.4172/2327-5073.C1.028
Abstract
Mycoplasma genitalium is a pathogenic bacterium that lives on the urinary and genital tracts in humans and can cause significant morbidity in men and women. Related infections are often asymptomatic. There are potentially serious complications that can result from an untreated infection. Routine screening of high-risk individuals would be critical for the detection and treatment of M. genitalium. In the absence of adequate and reliable culture and approved commercial assay techniques, most laboratories use in-house nucleic acid amplification tests for detection of this bacterium. This study aimed to develop a sensitive molecular method of PCR -ELISA for detection of M. genitalium and alternatively determine the prevalence of infection in women referred to a referral infertility Center in Mashhad, Northeast of Iran. Sampling process was done using vaginal swab from 100 infertile women and 100 fertile women as a control group. Isolation of M. genitalium DNA from clinical samples was performed using DNA extraction kit. Primers and biotinylated probe was designed for amplification of MgPa and 16S rRNA genes. For labeling PCR products, we used digoxigenin labeled nucleotides. Hybridization was done in streptavidin coated plate and detection was carried out by ABTS substrate. In this study, prevalence of M. genitalium in infertile women and pregnant women was 21% and 3.3%, respectively; which was statistically significant (p=0.024). Due to high sensitivity and cost effectiveness of PCR- ELISA method in detection of M. genitalium infection, this method can be used as an efficient screening method for diagnosis of this infection routinely.
Biography

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version