Immunome Research
Open Access
ISSN: 1745-7580
+44-20-4587-4809
ISSN: 1745-7580
+44-20-4587-4809
Maria Infantino
Florence Health Agency Hospital, Italy
Posters & Accepted Abstracts: Immunome Res
Background: Gluten is the target of several diseases such as wheat allergy (WA), celiac disease (CD) and non celiac gluten sensitivity (NCGS). NCGS is a new clinical entity characterized by gastrointestinal and extraintestinal symptoms comparable to those of CD patients but to date still lacking of specific biomarkers so that NCGS diagnosis can be reached only by excluding CD and WA and based on the direct association between gluten ingestion and symptoms onset. Previous studies showed that anti-gliadin antibodies (AGA) IgG are the most prevalent positive antibodies in NCGS population. Aim: The first aim of the study was to estimate AGA distribution and prevalence in a NCGS population. The second aim was to identify a serological pattern to help the diagnosis and or to mark the NCGS disease. Methods: Sera from 59 patients with suspected NCGS, 90 CD patients and 70 healthy individuals were assessed for IgG/IgA AGA, IgG/IgA deamidated gliadin peptide antibodies (DGP-AGA), IgA tissue transglutaminase antibodies (tTGA) and IgA endomysial antibodies (EmA) and HLA typing (Eurospital, Trieste, Italy). Results: We evaluated data by a dual statistical approach; Logistic Regression and Receiver Operating Characteristic (ROC) analysis; therefore, we showed a poor diagnostic accuracy of AGA IgA and IgG in NCGS condition. Conclusion: Our preliminary data showed that IgG AGA did not seem to be an adequately sensitive marker, even if it has been recently proposed as promising marker for NCGS condition, together with negativity for other celiac disease related antibodies. More in-depth clinical and laboratory researches are mandatory.