alexa Differential Expression Of SOCS1/SOCS3 Ratios In Virus-infected Macrophage Cell Lines | 19565
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Clinical & Cellular Immunology

Nancy J Bigley
ScientificTracks Abstracts: J Clin Cell Immunol
DOI: 10.4172/2155-9899.S1.017
Abstract
Herpes simplex virus type 1 (HSV-1) infection was examined on murine J774A.1 and RAW 264.7 macrophage cell lines unpolarized (M0) or polarized by IFN-γ and or cytokines to either an M1 (pro inflammatory) or M2 (immunomodulatory and tissue remodeling) phenotype. Dengue virus2 (DENV2) infection of RAW264.7 cells was also investigated. Morphology, cell viability, expression of cell surface markers, expression ratios of suppressor of cytokine signaling molecules SOCS1/ SOCS3, and capacity to replicate HSV-1 were determined. Morphological differences were abrogated by virus infection. Infection with either virus diminished expression of CD14 and CD86 in M1 populations, cells which exhibited significant decreases (p<0.001) in cell viability at 24 hours post infection. Western blotting suggested that SOCS1/SOCS3 expression ratios differed between uninfected and HSV-1-infected RAW264. 7 M1 cells, while ratios in the uninfected cells and the DENV2-infected M1 cells remained similar. Flow cytometry demonstrated that this ratio between uninfected M1 cells and HSV-1-infected M1 phenotypes fell from 6:1 to 1:1 in M1 phenotypes of J774A.1 cells. SOCS3 expression increased 7-fold in the M1 infected J774A.1 cells compared to uninfected M1 cells. Three- to four-fold decreases in HSV-1 yield occurred by 24 hours after infection in the M1 subpopulations. This decrease may reflect the anti-inflammatory effect of the relative increases in S0CS3 expression in these cells and/or the decreased survival of M1 cells after virus infection. Increases in SOCS3 expression by theHSV-1-infected M1 populations suggest a shift in the cell?s ability to respond to virus, a change not seen in DENV2 infectio
Biography
Nancy J Bigley received the BS degree in bacteriology from Penn State University in 1953 and the PhD from The Ohio State University in 1957 and has taught immunology and microbial pathogenesis at The Ohio State University, Chicago Medical School and Wright State University. She has published more than fifty papers in the field and authored two editions of a textbook in immunology. Her areas of research involve viral and cellular immunity and more recently innate immunity at the cellular level.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7