alexa Differential MiRNAs Expression Profiles Classify Mucin 1(+)/(-) Human Breast Cancer Stem Cells | 62418
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

14th World Cancer & Anti-Cancer Therapy Convention

Madhulika Singh and Yogeshwer Shukla
Indian Institute of Toxicology Research-CSIR, India
ScientificTracks Abstracts: J Cancer Sci Ther
DOI: 10.4172/1948-5956.C1.093
Abstract
Background: Mucin 1 (Muc1) is a secreted, oncogenic mucin that is aberrantly overexpressed in breast cancer cells but its potential role in breast cancers stem cells (BCSCs) have not been explored. MicroRNAs (miRNA), small noncoding RNAs that play critical roles in normal stem cell functions during development, have emerged as important regulators of BCSCs as well. Methods: Muc positive (+)/(-) cells were isolated from patient-derived cancer (n=25), and normal BC tissues (n=15) and propagated in non-adhesive suspension cell culture to assess their phenotypic characteristics. Further miRNAs expression profiling was done by using microRNA TaqMan® Low Density Array Cards v2.0 (TLDA cards A, Invitrogen), based on qReal-Time PCR array. Results: Significantly altered expression of miRNAs were found (17 upregulated and 29 down regulated) in Muc (+) BCSCs as compared to Muc(-) (p<0.05). All these miRNAs were having significant role in BCSCs self-renewal, proliferation potential and were also involved in cancer metastasis. Further, selected miRNAs expression levels were individually tested and validated in mammospheres generated from tissue samples. Muc(+) BCSCs were showing higher level of miRNAs -9, 16, 34a, 195-5p and 454 as compared to Muc(-) BCSCs. Significantly downregulated expression of miR-106a, 125b and 218 was also noted in Muc (+) BCSCs as compared to adhered and Muc(-) cell population. Conclusions: These miRNAs can potentially be used to develop a panel for classification and prognosis in order to better predict the progression of the disease and facilitate the choice of treatment strategy.
Biography

Madhulika Singh has completed PhD in the year 2003 from University of Lucknow (India) and Postdoctoral studies from CSIR-Indian Institute of Toxicology Research, Lucknow (India). Presently, she is working as Faculty in Department of Zoology at Maharishi University of Information Technology, Lucknow, India. She has published more than 36 research papers, review articles and book chapters in reputed journals and also been serving as an Editorial Board Member and Reviewer in many journals. Her research is based on understanding the self-renewal and differentiation capacities of cancer stem-like cells. Along with this, she is also engaged in discovering the scientific basis for anticancer activities of phytochemicals.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7