Journal of Microbial & Biochemical Technology

Differentiation of Listeria monocytogenes strains base on multi complete gene sequence

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Yong Zhao, Qingchao Xie, Haiquan Liu, Jing Xie and Jie Ou

Shanghai Ocean University, China

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

The Gram-positive bacterium Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne infection. A multi complete gene sequencing scheme was developed for accurate, and sensitive subtyping L. monocytogenes. Complete DNA sequences for six virulence genes (prfA, plcA, hly, mpl, actA, and plcB) and two housekeeping genes (prs and ldh) in eight American Type Culture Collection (ATCC) L. monocytogenes strains with seven serotypes were sequenced and analyzed. Results showed multiple DNA sequence alignment identified 8 (prs), 5 (prfA), 8 (plcA), 8 (hly), 8 (mpl),8 (actA), 7 (plcB), and 8 (ldh) allelic types and a total of eight unique sequence types. mpl was the most polymorphic gene and the phylogenetic tree based on its sequence differentiated four groups with high bootstrap values. The mpl and ldh genes appear to be good molecular markers for further epidemiological investigation of L. monocytogenes. A combined virulence gene and the housekeeping genes multi complete DNA sequences scheme will be a promising method enhanced discriminatory power for L. monocytogenes. Recent Publications 1. Xiao Shen, Qingchao Xie and Yong Zhao (2014) Antimicrobial effect of blueberry (Vaccinium corymbosum L.) extracts against the growth of Listeria monocytogenes and Salmonella enteritidis. Food Control 35:159-165. 2. Jing Jing Wang and Yong Zhao (2014) Fate of Vibrio parahaemolyticus on shrimp after acidic electrolyzed water treatment. International Journal of Food Microbiology 179:50-56. 3. Zhaohuan Zhang and Yong Zhao (2015) Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, Listeria monocytogenes and Salmonella spp. in raw shrimp. Food Control 51:31-36. 4. Lili Xiao and Yong Zhao (2015) Development of a quantitative real-time PCR assay for viable Salmonella spp. without enrichment. Food Control 57:185-189. 5. Sun Wenshuo, Zhao Yong and Wu Aibo (2015) A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods. Journal of Chromatography A 1387:42-48.

Biography :

Yong Zhao obtained his Doctorate of Science in Microbiology at the Nanjing Agricultural University in 2005. In 2007, he was selected for the young pioneer project of Shanghai College of Science and Technology, and he was selected for the “ShuGuang project" in Shanghai in 2015. His main research areas are Molecular Ecology of Food Microbiology, Food Quality and Safety Risk Assessment and Food Quality Safety and System Biology Research. He has hosted more than 20 scientific research projects, including the National Natural Science Foundation of China, and participated in more than 10 scientific research projects.