alexa Direct Detection Of Lead In RTIL Using DPASV On BDD Film Microcells And Determination Of Concentration Factor After Extraction From Aqueous Samples
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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In Association with
 
romanian
 
icamb2015

3rd International Conference and Exhibition on Biosensors & Bioelectronics
August 11-13, 2014 Hilton San Antonio Airport, San Antonio, USA

Amel Sbartai, Amani Chrouda, Philippe Namour, Nicolas Sarrut, Jan Krejci, Radka Kucerova and Nicole Jaffrezic-Renault
Accepted Abstracts: J Biosens Bioelectron
DOI: 10.4172/2155-6210.S1.022
Abstract
European Water Framework Directive predicted non effect concentrations for water organisms require determination of lead at very low concentrations: 1.2 μg/L. These low concentrations, generally in complex sample matrixes, have influence on the sensitivity and accuracy of the analytical method. Hence, prior to determination, a clean-up and/or enrichment step is highly necessary. In this work, for the first time, the determination of Pb was performed using Differential Pulse Anodic Stripping Voltammetry (DPASV) on a boron-doped diamond microcell directly in the room temperature ionic liquid (RTIL) extracting phase: Butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide which contained the complexing agent Trioctylphosphineoxide (TOPO). The calibration curves with and without TOPO are linear in the concentration range 0-4 μg/L of Pb, with a detection limit (DL) of 0.3 μg/L. The optimum conditions for higher concentration factor were determined: the aqueous phase should be a 0.1 M citrate buffer with pH2. The obtained concentration factor was 5.0 ?0.2 for lead in RTIL without chelating agent TOPO, and 9.0? 0.10 in IL with chelating agent TOPO.
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