alexa Discovery And Validation Of Syndecan2 Methylation For Serum DNA-based Test For Early Detection Of Patients With Colorectal Cancer And Precancerous Lesions
ISSN: 2161-1025

Translational Medicine
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

3rd International Conference on Translational Medicine
November 03-05, 2014 Las Vegas, USA

Sungwhan An
Accepted Abstracts: Transl Med
DOI: 10.4172/2161-1025.S1.014
A proper screening for colorectal cancer (CRC) can reduce morbidity and mortality by this disease. However, the current screening modalities are inadequate due to high cost and poor compliance leading to a low participation rate. Therefore, it would be urgently necessary to develop an alternative screening tool such as biomarker-based blood test.We have attempted identify DNA methylation sites which can be utilized as a biomarker for the non-invasive diagnostics of CRC patient at earliest stage.For this, we utilized methylation microarray analysis coupled with enriched methylated DNA bymethylated DNA isolation assay (MeDIA) and discovered differentially methylated genes between tumor and non-tumor tissues.Through step-wise filteringprocedures we then selected an aberrant SDC2 methylation as a potential biomarker for discriminating CRC patients from healthy individuals. When we analyzed methylation status of SDC2 in tumor and non-tumor samples by pyro-sequencing we found that abnormal SDC2 methylation is somatically acquired in the majority of patients with CRC (98%, n=133).We then revealed that the quantitative SDC2 methylation (qMSP) test by real-time PCR in serum DNA can successfully detect of various stage of CRC patients (n=131)with overall high sensitivity (87%, n=131)and specificity (95% n=125). Notably,the sensitivity was 92% for stage I.Currently we extended this to investigate whether aberrant presence of SDC2 methylation in blood can also distinguish patients with adenoma from normal subjects. For quantitative SDC2 methylation assay in heterogeneous and minute amount of serum DNA from precancerous patientswe have optimized detection method of qMSP (meSDC2 IP-qMSP) by degenerated inosine-primer design. The limit of detection for methylated SDC2 test was 100 pg/mL of serum corresponding to ~30 genome copies.A meSDC2 IP-qMSP in serum DNA from independent group of CRC patients(n=146) with various stages (I-IV), 100 adenoma patients and 51 colonoscopy-verified normal subjects showed the overall sensitivities of 91.1% (95% CI, 85.3% to 95.2%) and 71.0% (95% CI, 61.1% to 79.6%) for CRC and adenomatous polyps, respectively with a specificity of 86.3% (95% CI, 73.7% to 94.3%). The sensitivity was 93.5% (29/31) for stage I of CRC.SDC2 methylation level in serum from preoperative patients dramatically disappeared or dropped to lower after curative surgery (P=0.002) indicating this phenomenon is a CRC-specific. We demonstrated that a simple blood DNA test that measures an abnormal presence of SDC2 methylation in serum DNA can successfully distinguish not only CRC patients but also those with adenoma from healthy subjects.
Sungwhan An is the Founder and Chief Executive Officer of Genomictree, Inc.; molecular diagnostic company based in South Korea since 2000. He is an Adjunctive Professor in Cancer Center of Yonsei University, College of Medicine, South Korea and also had been worked as aResearch Assistant Professor from 2003 to 2009. After that he earned PhD degree in the field of molecular virology at the University of Texas at Austin in U.S.A. He then went through a Postdoctoral training course involved in translational research work investigating transcriptomics in HCV infection using DNA microarray at Dr. Harry Greenberg?s lab in the Stanford University School of Medicine from early 1999 to late 2000. Currently his interest is to discover and develop new DNA methylation biomarkers for the detection of cancers in non-invasive way. In addition, he has been focusing to develop multiplex assay technique for the multiple markers including multiple cancer mutations and infectious agents.
image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

1-702-714-7001Extn: 9042

General Science

Andrea Jason

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001Extn: 9042

© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version