Journal of Bacteriology & Parasitology

E. coli seroprevalence and antibiotic resistance-A study from patientsâ?? visiting Tribhuvan University Teaching Hospital, Nepal

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Tarani Prasad Paneru

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

E. coli are used as the ideal indicator to distinguish and measure fecal contamination in the assessment of food and water
safety. Pathogenic E. coli strains are distinguished by their ability to cause serious illness from other E. coli. As per the
study total 450 isolates of E. coli were obtained from different clinical samples from TUTH laboratory, predominantly were
from urinary tract diseases and other abscesses isolated from diverse infections. The study was accomplished from January
1999 to March2001.To come across the existing passé antibiotic resistance pattern of E. coli. The study was carried out at
TUTH laboratory with the objectives to discover the prevalence of different sero groups of E. coli along with to calculate the
significance antibiotic resistance correlation between serogroups and non serogroups of isolates. E. coli, such as O157:H7,
O121, O103, O91, O104, O111, O113, O117, O118, O128 and O145, were the serotypes isolated from different clinical samples.
Antimicrobial susceptibility testing of the isolates was performed using the Kirby–Bauer disc diffusion method and Mueller–
Hinton agar. The result was found that all isolates were resistant to ampicillin. Overall (80%) were resistant to tetracycline and
cephalexin (60%) to co-trimoxazole and (40%) to gentamicin even though most of the isolates were sensitive to ciprofloxacin.
Sixty percent of the isolates were resistant to three antibiotics and 40% to four antibiotics. It was found that among the eight
antibiotics six antibiotics namely Cefixime, Nalidixic Acid, Ciprofoxacin, Nitrofurantoin, Amikacin and Imipenem were
significantly resistance to different serotypes where as only two antibiotics Ampicillin and Gentamycin were significantly no
difference with serotypes. (p=0.05).Different PCR assays were used to distinguish virulence factors, sero groups and antibiotic
resistance genes in the E. coli isolates. The entire PCR yields were electrophoresed on 1.5% agarose gels that were strained with
ethidium bromide and scrutinized under ultraviolet illumination.