alexa Effect Of Different Nannochloropsis Oculata Diets (cultured Under Different Nitrogen And Phosphorus Regime) On Growth And Biochemical Composition Of Rotifers Brachionus Plicatilis
ISSN: 2471-9315

Applied Microbiology: Open Access
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12th International Congress on Microbial Interaction and Applications of Beneficial Microbes
July 17-18, 2017 Munich, Germany

Nour A M, M A Zaki, M Ashour, Abd El-Wahab Kamil, Taha S M and Eglal Omar
Alexandria University, Egypt
National Institute of Oceanography and Fisheries, Egypt
Posters & Accepted Abstracts: Appli Micro Open Access
DOI: 10.4172/2471-9315-C1-006
Abstract
Microalgae play an important role in aquaculture as a means of enriching zooplankton for feeding fish and other larvae. A previous series of incubations were conducted in order to investigate the effect of different molar concentrations, sources and ratios of nitrogen (as urea or ammonium nitrate) and phosphorus (as phosphoric acid) of commercial agricultural fertilizers (CAGF) on growth and biochemical compositions of N. oculata. The incubations achieved microalga N. oculata with different chemical composition as following: highest lipid percentage (HL), lowest lipid (LL), highest protein (HP), lowest protein (LP), highest carbohydrate (HC) and lowest carbohydrate percentage (LL), that comparing to the seventh patch of F/2 standard Guillard medium as a control (CO) treatment. The obtained N. oculata from the seven incubations were served as a live food for rotifer Brachionus plicatilis reared in batch culture for 72 hours in order to examine its effects on growth performance (population, population growth rate, fecundity, filtration and ingestion rate) and biochemical composition (lipid, protein, carbohydrates, fatty acids and amino acids profile). The results showed that there were no significant differences (P≤0.05) in population (RFn) and population growth rate (Rr) of rotifer, whereas significant differences (P≤0.05) were achieved by rotifer fecundity (RFc), filtration (RF) and ingestion rate (RI). The highest RFn (156±8.49 Ind. mL-1), Rr (0.0158 Ind./day) and RFc (0.405±0.005 egg/popu.) were achieved by rotifer fed on LL N. oculata diet. On the contrary, the lowest RFn (116±1.41 Ind./ml), Rr (0.0117 Ind./day) and RFc (0.211±0.004 egg/popu.) were achieved by rotifer fed on HC N. oculata diet. On the other hand, rotifer fed on N. oculata diet cultured on F/2 medium (CO) achieved the lowest significant rotifer carbohydrates percent (10.94%). The highest significant total protein percentage (46.53%) and energy (665270 ca./g) of rotifer were achieved by rotifer fed on N. oculata diet LL. On the other hand, rotifer fed on diet HC achieved the highest rotifer lipids (35.56%) and carbohydrates (18.88%), respectively. Finally, our result demonstrated that the microalga N. oculata cultured on CAGF under different nutrient limitation achieved high significant rotifer B. plicatilis growth and biochemical composition when compared, as rotifer diets, to F/2 standard media, with advantage of reduced cost media.
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