alexa Effect Of Growth Regulators On The Production Of Thymol In Suspension Cultures Of Nigella Sativa Linn
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference on Pharmacognosy, Phytochemistry & Natural Products
October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Hera Chaudhry, Nida Fatima and Iffat Zareen Ahmad
Accepted Abstracts: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.004
Abstract
Nigella sativa is an extensively studied plant for its medicinal properties which are attributed because of the active constituents like thymoquinone, thymol, p-cymene, pinene, dithymoquinone etc. Present study deals enhanced production of thymol (2-isopropyl-5-methylphenol) a natural monoterpene phenol in the suspension culture of the Nigella sativa cultured in Erlenmeyer flasks (250 ml) placed in an incubator shaker at 110 rpm and at 25?1?C with a 16 hr photoperiod each containing 100 ml of Murashige & Skoog medium. Friable, soft and white calli of epicotyl region of the in vitro grown seedlings were used for the initiation of suspension cultures. Different combinations and concentrations of the growth hormones were tried to elucidate their effect on the thymol production. Presence of thymol was detected by employing one dimensional thin layer chromatography with toluene:chloroform:etanol (40:10:10) as mobile phase and keeping thymol as standard. Results showed the culture supplemented with KN (2 ppm) +NAA (1 ppm) gave a large pink spot with Rf value of 0.74 as compared to the standard and negative control which gave a small pink spot with Rf value 0.75 and diminished spot with Rf value 0.70 respectively. Therefore, this study can be efficiently used to specifically produce and enhance the production medicinally important compound thymol by employing plant tissue culture techniques.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version