Inflammatory factors are important contributors to pathogenesis of cardiovascular diseases.
Activated monocytes and neutrophils excrete enzyme myeloperoxidase, which catalyses
production of hypochlorous acid (HOCl) from H
. HOCl is unstable compound
and strong oxidizing agent affecting plasma lipoproteins, fibrinogen and other proteins. Their
alterations have effect on development of atherosclerosis and thrombosis complications.
Platelets actively participate in these processes. The published data about HOCl effects on
platelet aggregation are inconsistent.
The aim of this study was to determine the dependence of effects of hypoclorous acid on the
intensity of platelet aggregation
in healthy people and heart failure patients on HOCl
: Two groups of people were investigated: healthy people (n=10) and heart failure
patients (n=18). Platelet aggregation was determined with optical 2-channeled Chrono-log
platelet aggregometer. The standard method of changes of relative optical density in aggregating platelet-rich plasma against platelet
poor plasma was used. The aggregation was initiated with ADP (3,8 mmol/L) solution. We used 6 samples of the same person
plasma. To each sample 10 μL HOCl of different concentrations were added: 2.12 mM/L, 7.06 mM/L, 10.59 mM/L, 21.18 mM/L, and
43.4 mM/L as final concentration. To the control sample the same volume of physiological solution was added. The samples were
incubated for 30 min at 37�C, and the intensity of platelet aggregation using ADP was determined (%).
It was determined that high concentrations of HOCl (from 2.12 to 43.4 mM/L) significantly decreased platelet aggregation
in both groups. We observed that the icreasing concentration of HOCl was reversely proportional to the intensity of platelet
aggregation. The decrease of platelet aggregation intensity in plasma of heart failure patients affected with HOCl
expressed than in healthy people (42.6% and 36.1% respectively).
The intensity of platelet aggregation significantly decreased dependently on HOCl concentration in plasma
Heart failure patients were more affected than healthy people
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