Applied Microbiology: Open Access
Open Access

Effect of nutrient limitations on growth and biochemical composition of outdoor cultured Chaetoceros calcitrans

12^th International Congress on Microbial Interaction and Applications of Beneficial Microbes

July 17-18, 2017 Munich, Germany

Eglal Omar, M A Zaki, M Ashour, Abd El-Wahab Kamil, Taha S M and Nour A M

Alexandria University, Egypt
National Institute of Oceanography and Fisheries, Egypt

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

Chaetoceros calcitrans is the most commonly grown microalgae in shrimp and bivalve hatcheries. Optimizing the suitable culture media for mass culture of C. calcitrans is very important in the industrial production scale. Our study aims to improve the quality and quantity C. calcitrans, cultured outdoor under sunlight, by using economical and convenient media prepared from commercial agricultural fertilizers (CAGF), compared to F/2 medium. However, we tested 9 different nutrient medium belonging to three nitrogen groups (each group have three nitrogen molar concentration (50%, 100%, and 150%) related to molar concentrations of nitrogen in F/2 Guillard medium); sodium nitrate (N50, N100 and N150), urea (U50, U100 and U150) and ammonium nitrate (A50, A100 and A150) with the equivalent F/2 phosphorus molar concentration but in phosphoric acid form. The results of the present study found that there were significant differences (Pâ�?¤0.05) between all treatment media. The highest significant (Pâ�?¤0.05) dry weight (0.859 g/l) and cell density (7.882 106 cell/ml) were achieved by A150 while the lowest (0.0.644 g, 1.156 106 cell/ml) was achieved by N50. The highest protein (49.99%) was achieved by A150, while the lowest protein (43.11%) was achieved by A50. Moreover, A50 achieved the highest lipid percentage (17.08%), as well as the lowest protein (43.11%), while the lowest lipid (7.69%) was achieved by N100. Lipid productivity (ProLip) was ranging from 0.010 to 0.028 (g/l/d), however, the highest ProLip was achieved by A50 (0.028 g/l/d), while the lowest was achieved by nitrogen-sodium nitrate (0.008 to 0.010 g/l/d). Finally, the results concluded that CAGF should be used at mass production scale to reduce production cost and to enhance quality and quantity of C. calcitrans in marine shrimp hatcheries.

Biography :

Email: dreaio2015@yahoo.com