Biochemistry & Pharmacology: Open Access
Open Access

Effect of quercetin on tight junctional proteins and BDNF signaling molecules in hippocampus of PCB exposed adult male rats

International Conference on Pharmacognosy, Phytochemistry & Natural Products

October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

K. Selvakumar, S. Bavithra, E. Sugantha Priya, A. Anita Roy, P. Daisy and J. Arunakaran

Accepted Abstracts: Biochem & Pharmacol

Abstract :

Polychlorinated biphenyls induced ROS alters blood brain barrier integrity which is paralleled by tight junctional proteins. Brain derived neurotropic factor plays an important role in the maintenence, survival of neurons and synaptic plasticity. It is predominant in the hippocampal areas vital to learning, memory and higher thinking. Quercetin had drawn attention to the neurodefensive property. We aimed to study the role of quercetin on serum PCB, estradiol, testosterone levels and mRNA expressions of estradiol alpha and beta, expressions of tight junctional proteins, BDNF signaling molecules on hippocampus of PCB exposed adult male rats. Rats were divided into four groups: Group I: Vehicle treated (i.p); II. quercetin 50 mg/kg bw (gavage): III. PCB (Aroclor 1254-2 mg/kg/bw/ip) treated and IV. quercetin 50 mg/kg/bw (gavage) simultaneous PCB same dose (ip). All treatments were for 30days/daily/ number of animals 6 each (adult albino male). 24h treatment animals were sacrificed, hippocampus was separated. Blood collected and sera were separated and analyzed for serum PCB (GC MS), testosterone and estradiol (RIA) and hippocampal mRNA epressions of tight junctional proteins, BDNF signaling molecules were studied by RTPCR. All the parameters were decreased, quercetin treatment brought back to normal. The data will be discussed with the available literature.