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|Nam Mi Kang, Won-Ho Hahn, Seunghyun Song, Suyeon Park and Jaehan Kim|
|Department of Nursing, Konkuk University, Chungju, Republic of Korea
Department of Pediatrics, School of Medicine, Soon Chun Hyang University, Seoul Hospital, Republic of Korea
Department of Political Sciences, Faculty of Social Sciences, University of Antwerp, Belgium
Department of Biostatistics, Soon Chun Hyang Medical Center, Seoul, Republic of Korea
Department of Food and Nutrition, colleague of Human Ecology, Chungnam National University
|ScientificTracks Abstracts: J Nurs Care|
|Background and Objectives: Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic
development of neonates. Moreover, freeze-drying seems to be promising storage method for better functions of human milk banks.
However, the effects of pasteurization and freeze-drying on HMOs were not evaluated yet. The purpose of this study is to analyze and
compare HMOs profiles before and after the pasteurization and freeze-drying.
Methods and Study Design: Totally nine fresh human milk samples were collected from three healthy mothers at first, second,
and third week after delivery from each mother. The samples were treated with Holder pasteurization and freeze-drying. HMOs
profiles were analyzed by MALDI-TOF/TOF mass spectrometry and compared between samples before and after the treatments by
Results: Human milk samples showed significantly different HMO patterns between mothers. However, HMOs were not affected by
lactation periods within 3 weeks after delivery (r2=0.972 ~ 0.999, P <0.001). Moreover, both of pasteurization and freeze-drying were
found not to affect HMO patterns in a correlation analysis (r2=0.989 ~ 0.999, P <0.001).
Conclusion: HMOs were found not to be affected by pasteurization and freeze-drying. In addition, there were large differences
between mothers. As significant changes of HMOs were not found after these treatments, we hope that introducing freeze-drying in
human milk banks would be supported by the present study. However, the storage length without composition changes of HMOs after
freeze-drying needs to be evaluated in the further study.
Running Title: Effects of pasteurization and freeze-drying on HMOs
Keywords: Freeze-drying, Human milk, Mass spectrometry, Oligosaccharides, Pasteurization
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2015R1A2A1A15056046)
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