alexa Efficacy Of Combination Chemotherapy With Clofazimine And Diminazene Aceturate Against Babesia And Theileria Infections
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th International Conference on Parasitology
September 01-02, 2017 | Prague, Czech Republic

Ikuo Igarashi
Obihiro University of Agriculture and Veterinary Medicine, Japan
ScientificTracks Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597-C1-034
Abstract
In the present study, we evaluated the growth-inhibitory effects of clofazimine, currently used for treating leprosy, against Babesia bovis, B. bigemina, B. caballi, and Theileria equi in vitro culture, and B. microti in mice. The IC50 values of clofazimine against the in vitro growth of B. bovis, B. bigemina, B. caballi, and T. equi were 4.5, 3, 4.3, and 0.29 μM, respectively. In mice infected with B. microti, treatment with oral administration of 20 mg/kg clofazimine resulted in a significant lower peak parasitemia (5.3%) as compared to a control group (45.9%), which was comparable to subcutaneous administration of 25 mg/kg diminazene aceturate. However, the growth of parasites was observed in mice after blood transfusions from clofazimine-treated mice on day 40 post-infection when parasites were not found in the blood smears. These results suggest that clofazimine has excellent inhibitory effects against Babesia and Theileria in vitro and in vivo, but it could not completely eliminate parasites in the host. Therefore, we evaluated the combination treatment with clofazimine and diminazene aceturate against piroplasms both in vitro and in vivo for the development of a novel chemotherapy with high efficacy and safety against animal piroplasmosis. The clofazimine-diminazene aceturate combination showed additive or synergistic effects on in vitro growths of Babesia bovis, B. bigemina, B. caballi, and Theileria equi. The lower dosages of clofazimine-diminazene aceturate combination showed higher chemotherapeutic efficacy against B. microti in mice as compared to clofazimine or diminazene aceturate monotherapy. B. microti was not detected in blood, brain, spleen, and heart DNA samples by PCR from combination therapy on day 51 post infection. Furthermore, the parasite did not grow in blood-transfused mice from combination therapy groups. All results suggest that the clofazimine-diminazene aceturate combination chemotherapy will be a better choice to treat animal piroplasmosis instead of diminazene aceturate mono chemotherapy.
Biography

Ikuo Igarashi is currently a Professor at National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Japan and an OIE expert for equine piroplasmosis and bovine babsiosis. He has done researches on the in vitro cultivation of Babesia and Theileria and its application for drug screening. In addition, he is investigating the molecular mechanisms of invasion and multiplication of Babesia parasites in red blood cells and the development of highly specific and sensitive serological and molecular diagnostic assays. He has published more than 250 parasitology papers in peer-reviewed scientific journals, book chapters, and patents. He has been also serving as a member of the Editorial Advisory Board for Veterinary Parasitology since 2005 and an Academic Editor of PLoS ONE from 2012 to 2015. He is currently a Co-Editor-in-Chief for Veterinary Parasitology.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords