alexa Efficient Expression Of Porcine Circovirus Type 2 Virus-like Particles In Escherichia Coli
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

15th Annual Summit on Vaccines and Immunization
February 20-21, 2017 Berlin, Germany

Guanggang Qu
Shandong Binzhou Animal Science and Veterinary Medicine Academy, China
ScientificTracks Abstracts: J Vaccines Vaccin
DOI: 10.4172/2157-7560.C1.054
Abstract
Porcine circovirus 2 (PCV2) is considered to be the etiologic agent responsible for porcine circovirus associated disease (PCVAD) that frequently affects growing pigs at 5-18 weeks of age and can lead to significant negative impacts on profitability of pork production. The capsid (Cap) protein of PCV2 is a major candidate antigen for development of recombinant vaccine and has been successfully used as a vaccine to control porcine circovirus associated disease (PCVAD). In our study, full-length ORF2 gene with codon-optimized for E. coli was synthesized and inserted into the PTF9 (+) expression vector to improve expression of recombinant Cap protein (rCap). A large amount of soluble rCap protein was obtained. The recombinant Cap protein expressed by Escherichia coli (E. coli) has the ability to self-assemble into virus-like particles (VLPs) in vitro; it is particularly an opportunity to develop the PCV2 VLPs vaccine in E coli. In this report, a highly soluble Cap-tag protein expressed in E coli was constructed with a p-TF9 expression vector with a fusion tag of TF9. The recombinant Cap was purified using Ni2+ affinity resins and the tag was removed by the TEV protease. Simultaneously, the whole native Cap protein was able to self-assemble into VLPs in vitro when viewed under an electron microscope. The Cap-like particles had a size and shape that resembled the authentic Cap. The result could also be applied in the large-scale production of VLPs of PCV2 and could be used as a diagnostic antigen or a potential VLP vaccine against PCV2 infection in pigs. We have, for the first time, utilized the Fh8 fusion motif to successfully express the complete Cap protein of PCV2 in E coli. After the cleavage of the fusion motif, the nCap protein self-assembled into VLPs, which can be used as a potential vaccine to protect pigs from PCV2-infection.
Biography

Guanggang Qu is interested in Preventive Veterinary Medicine, Veterinary Biotechnology and Biological Products. He is particularly interested in developing innovated vaccines by E. coli expression system. Currently, he is an Assistant Professor at Shandong Binzhou Animal Science & Veterinary Medicine Academy and Vice Director at Shandong Binzhou Research and Development Centre of propolis vaccine for livestock and poultry. He was a Visiting Scientist at Bhabha Atomic Research Centre and at U.S. Department of Agriculture of USA from 2011 to 2013. He completed his Doctoral Degree at Jilin University, China.

Email: [email protected]

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords