Journal of Bacteriology & Parasitology

Emergence of OXA-48 Carbapenem-resistant Enterobacteriaceae (CRE) among paediatric cancer patients in Egypt

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Dina Mohamed Osama

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

The spread of CRE species causing both nosocomial and community acquired infections, has become a worldwide major
public health problem. Carbapenems are considered the last line of defense against multidrug-resistant gram-negative
organisms; resistance to these agents reduces the treatment options that further lead to treatment failure. As a result, severe
infections with CRE are associated with significant morbidity, and mortality. Therefore, there is an urgent need for rapid and
sensitive methods for screening and identifying carbapenemase producers among carbapenem resistant isolates. A panel of 100
isolates from different clinical samples were collected in the Microbiology unit of the Children cancer hospital in Egypt. The
isolates were identified using different conventional methods and confirmed by the automated Vitek 2 system. Antimicrobial
susceptibility testing was performed using the Kirby Bauer disc diffusion method. Carbapenems MICs were determined by
the automated Vitek-2 system. Phenotypic tests used for carbapenemase producers detection were; the screening medium
ChromID Carba Smart, the RAPIDEC Carba NP test, and the Vitek 2 system.
Out of 100 isolates, 43 Escherichia coli, 50 Klebsiella pneumoniae, 3 Klebsiella oxytoca and 4 Enterobacter cloacae were
identified. The Vitek 2 system showed that 66% of the isolates were carbapenem resistant. Ninety percent of the detected CRE
isolates were classified as OXA-48 type and 10% only were classified as both KPC and NDM-1 carbapenemase producers.
Ninety percent of the resistant isolates gave positive results with the Carba NP test and 10% gave negative results. The spread
of this type of CRE isolates within this setting is very alarming and requires full attention and rapid implementation of strict
infection control measures.