alexa Emergence Of ST117 Enterococcus Faecium Carrying Both VanA And VanB Genes In Greece
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

International Conference on Medical and Clinical Microbiology
July 03-04, 2017 Bangkok, Thailand

E Petinaki, C Papagiannitsis and A Skoulakis
University Hospital/Medical School, Greece
University of Thessaly, Greece
Posters & Accepted Abstracts: J Med Microb Diagn
DOI: 10.4172/2161-0703-C1-009
Abstract
Statement of the Problem: Vancomycin-Resistant Enterococci (VRE) are recognized as an important nosocomial problem worldwide, posing a particular threat in healthcare facilities (HCF). On November 2016, one E. faecium, carrier of both vanA/ vanB genes, was isolated from the blood cultures of a patient in the University Hospital of Larissa, Thessaly, Greece. The isolate, Efa-125, exhibited resistance to both vancomycin and teicoplanin (MICvancomycin: 256mg/L, MICteicoplanin: 256 mg/L), and was also resistant to various antimicrobial agents except, linezolid, daptomycin and tigecycline. Based on Multi Locus Sequence Typing (MLST), Efa-125 belonged to ST117 clone (Clonal Complex 17). Given that, few strains worldwide, were found to carry both vanA and vanB genes, the strain was further analyzed regarding the genetic units carrying vanA and vanB genes. Methodology: The genomic DNA of E. faecium Efa-125 was extracted using the DNA-Sorb-B kit (Sacace Biotechnologies S.r.l., Como, Italy). Plasmids and chromosomes were sequenced using the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA). Initial paired-end reads were quality trimmed using Trimmomatic tool and assembled via de Bruijn graph-based de novo assembler SPAdes. The sequence gaps were filled by a PCR-based strategy and Sanger sequencing. For sequence analysis and annotation, the BLAST algorithm (www.ncbi.nlm.nih.gov/BLAST), the ISFinder database (www-is.biotoul.fr/), and open reading frame (ORF) finder tool (www.bioinformatics.org/sms/) were utilized. The nucleotide sequence of the plasmid pEfa- 125gr has been assigned GenBank accession number KY320277. Findings: The vanA gene was carried on a plasmid (29,320 bp) exhibiting high similarity to pA6981, previously characterized from E. gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into its chromosome. Conclusion & Significance: In Greece is the first detection of a vanA-vanB genotype/VanA phenotype E. faecium caused bacterieamia. Until now, vanA/vanB E. faecium strains are isolated in Middle East and express a VanB phenotype. This report indicates an evolving VRE epidemiology.
Biography

E Petinaki is a Clinical Microbiologist, Professor and Head of Department of Microbiology of the University Hospital/Medical School of University of Thessaly in Larissa (Central Greece). Her research interest is focused on the epidemiology of nosocomial infections, on the characterization of mechanisms of antimicrobial resistance and on the application of molecular methods for the identification of etiological agent of infectious diseases. She is responsible for the teaching of Microbiology in several Under-graduate and Post-graduate programs of Greek Universities and she is Coordinator in many national research studies.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version