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|Yuzhu Zhang and Hongfeng Chen|
|Longhua Hospital Affiliated to Shanghai University of TCM, China|
|ScientificTracks Abstracts: Biochem Pharmacol (Los Angel)|
|MDA-MB-231/DDP had higher IC50 value of DDP, lower intracellular DDP concentration, lower apoptosis ratio than MDAMB- 231 cell line treated with DDP. Considering the intracellular DDP concentration difference, we tested drug-resistant membrane proteins (MRP2, P-gp and BCRP), which were remarkably increased in MDA-MB-231/DDP cells. Next, we found these increased membrane proteins were induced by the activation of NF-κB pathway in MDA-MB-231/DDP cells. Furthermore, ETS1, RPL6, RBBP8, BIRC2, PIK3A and RARS were six important genes for DDP-resistance based on PPI network and expression validation. However, it has been reported enforced over-expression of ETS1 induced IKKα mRNA and protein expression as well as IKKα promoter activity. Our results suggested the protein expression of ETS1 and IKKα were significantly up-regulated in MDAMB- 231/DDP cells. In addition, inhibition of ETS1 expression enhances chemo-sensitivity to DDP and reversed the activation of NF- κB pathway in MDA-MB-231/DDP cells, whether ETS-1 binds to the core IKKα promoter and strongly induces its activity. Now, our team is researching the corresponding binding sites between ETS1 and IKKαby dual-luciferase and chromatin immunoprecipitation technique (ChIP).|
He is studying his PhD of medicine at Shanghai University of Traditional Chinese Medicine. His researches focus on key target genes of tumor prognosis, mechanisms of drug resistance and anti-cancer natural drugs.
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