The aim of this study was to evaluate the ability to detect human immunodeficiency virus HIV antibodies from DBS stored
C, and 50
C. To evaluate the uses of DBS for detection of HIV antibodies with rapid test, 50 HIV positive and
45 HIV negative DBS were tested with two rapid test (ACON ? One Step Test Device), and (ACCURATE ? test device), and to
evaluate the effect of storage temperature and time on sensitivity and specificity, DBS were tested after 2 weeks, 4 weeks, and 8
weeks. Sensitivity was ranged from 96-84%, and specificity was 100%. To detect the specificity and sensitivity of DBS stored for
12 weeks at 37
C, and 50
C, for HIV antibodies detection with ELISA kit, all 191 DBS were tested with (Anti-HIV 1 and
2 ELISA Diagnostic Kit, BIOREX DIAGNOSTICS ?), From 97 HIV-positive DBS, 96 gave positive result, while only one was
negative with sensitivity of 98.96 %. All 94 HIV negative DBS were negative at all temperature (Specificity 100 %).
HIV, DBS, ICT, ELISA, IgG, Sudan.
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