Basils are valuable multipurpose medicinal plants which belong to the family Lamiaceae and is extensively versatile group
consisting about 160 species. DNA markers are considered the best tools for determining genetic relationships/diversity as
they are unlimited in number, show high polymorphism and are independent of environmental interaction i.e. highly heritable.
RAPD technique has gained importance due to its simplicity, efficiency, relative ease to perform the assay and non-requirement
of DNA sequence Information. The RAPD assay was performed to assess the genetic diversity among six Ocimum species viz.
O. americanum, O. basilicum, O. canum, O. killimandscharicum, O. minimum and O. sanctum. The 18 decamer primers were
tested but only 8 primers namely OPO-02, OPO-07, POP-12, OPO-17, OPO-19, OPA-03, OPA-08 and OPA-10 gave amplifica
tion. Out of 61 RAPD bands 56 were polymorphic. The combination of RAPD data and the simple matching coefficient grouped
the species in clusters. The highest value of genetic similarity was observed between the O. basilicum and O. minimum (60%) and
lowest was recorded between O. basilicum and O. sanctum (27%). The PCA (Principle Component Analysis) was also performed
for their diversity assessment. The data derived from RAPD marker helped in a comprehensive understanding of the genetic
relationship among Ocimum species. This result provides valid guidelines for collection, conservation and characterization of
Ocimum genetic resources.
Nand Kishor Sharma has completed his M.Sc. (Agriculture Biotechnology) at the age of 24 years from Rajendra Agricultural University, Pusa and has
journals and has also presented some research papers in national conferences.
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