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|Igor Vivian Almeida|
|State University of Maringa, Brazil|
|ScientificTracks Abstracts: J Cancer Sci Ther|
|Breast cancer is associated with a high mortality rate around the world due to its aggressiveness and highly resistance to
conventional therapies. Sanguinarine (SAN) and chelerythrine (CHE) are plant alkaloids extracted from Sanguinaria canadensis
and Macleaya cordata, which have been studied for their bioactivities. In this study, it was observed that SAN was cytotoxic to human
breast adenocarcinoma cells (MCF-7) at concentrations of 7.5 μM (24 and 48 hours), effectively reducing cell viability from the
concentration of 10 μM for 24 hours and 7.5 μM for 48 hours, by the MTT test. CHE, in turn, was cytotoxic at concentrations of 10
and 20 μM (48 hours), but did not compromise the cellular viability. An analysis of the membrane integrity showed that both alkaloids
did not affect the cell viability by using the propidium iodide uptake. The comet assay analysis indicated that SAN was genotoxic to
the MCF-7 cells, with a significant increment of damage at 10 μM, while none of the tested concentrations of CHE showed a genotoxic
effect. The flow cytometry analysis indicated that no cell cycle arrest was caused by both alkaloids, but SAN 10 μM induced a sub-G1
population, due to a significantly higher percentage of apoptotic or necrotic cells. Thus, the results of cytotoxicity, genotoxicity, and
of the cell cycle monitoring that are presented in this paper have suggested that SAN has more of a chemotherapeutic activity, as well
as having the potential for the development of new therapies for breast cancer, when compared to CHE.
1. Almeida I V, Düsman E, Mariucci R G, Mantovani M S and Vicentini V E P (2014); Cytotoxicity and mutagenicity of fluoxetine hydrochloride (Prozac), with or without vitamins A and C, in plant and animal model systems; Genetics and Molecular Research. v.13, p.578 - 589.
2. Düsman E, Almeida I V, Lucchetta L and Vicentini V E P (2014); Effect of processing, post-harvest irradiation, and production system on the cytotoxicity and mutagenicity of Vitis labrusca L. juices on HTC Cells; Plos One. v.9, p.e107974.
3. Almeida I V, Domingues G, Soares L C, Düsman E and Vicentini V E P (2014); Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo; Brazilian Journal of Pharmaceutical Sciences. v.50, p.251 - 256.
4. Düsman E, Almeida I V, Coelho A C, Balbi T J, Tonin L T D and Vicentini V E P (2013); Antimutagenic Effect of Medicinal Plants Achillea millefolium and Bauhinia forficata in vivo; Evidence-Based Complementary and Alternative Medicine (Online). v.2013, p.1 - 6.
5. ALMEIDA I V, DÜSMAN E, HECK M C, PAMPHILE J A, LOPES N B, TONIN L T D, VICENTINI V E P (2013); Cytotoxic and mutagenic effects of iodine-131 and radioprotection of acerola (Malpighia glabra L.) and beta-carotene in vitro. Genetics and Molecular Research. v.12, p.6402-6413, 2013.
Igor Vivian Almeida is a Junior Professor and Researcher at the State University of Maringa, Brazil. His Doctoral thesis is under publication and has presented interesting insights on the anticancer activities of plant alkaloids, sanguinarine and chelerythrine, such as cytotoxicity, genotoxicity, cell cycle arrest and different ways of apoptosis induction on tumor and non-tumor cell lines. Some of his papers also present the effect of plant derivatives on chromosome aberration and genetic instability.
Email: [email protected]
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